ICC样细胞在正常豚鼠上尿路的分布及对平滑肌收缩的影响  

Distribution of interstitial cells of Cajal in normal guinea pig upper urinary tract and its effect on smooth muscle contraction

作　　者：钟晓[1] 李为兵[1] 方强[1] 宋波[1] 

机构地区：[1]第三军医大学西南医院全军泌尿外科研究所,重庆400038

出　　处：《第三军医大学学报》2009年第6期495-497,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30670776)~~

摘　　要：目的研究正常豚鼠上尿路不同部位起搏细胞(interstitial cells of Cajal,ICCs)的分布及其对局部平滑肌收缩的影响。方法取10只豚鼠分别取肾盂输尿管交界处、上、中、下段输尿管组织,经全层铺片处理后,行ICCs特异性标志物KIT免疫荧光染色,对各段组织进行细胞计数,单因素方差分析各段ICCs的分布及其差异;再于上述部位(除输尿管壁内段外)分别剪取2mm×6mm肌条进行张力测定实验,比较各部位肌条收缩频率及幅度的变化,分析其与ICCs密度变化的相关性。结果KIT染色阳性、形态与胃肠道起搏细胞ICCs相似的细胞(ICCs样细胞)存在于豚鼠上尿路的各段中,上尿路ICC样细胞密度在肾盂肾盏交界处、输尿管上、中、下段分别为(5.20±0.98)、(3.90±0.98)、(3.03±0.98)、(2.50±0.81)个/视野;各部位肌条收缩频率及幅度不同,但肾盂输尿管交界处、输尿管上段的肌条收缩频率及幅度较输尿管中段和下段明显增大,差异显著(P<0.05),输尿管中段和下段之间没有显著区别(P>0.05);相关性分析结果表明局部平滑肌肌条收缩频率和幅度与ICCs密度呈显著正相关性(P<0.05)。结论豚鼠上尿路平滑肌中存在ICCs,肾盂输尿管各部位ICCs的分布有显著差异,其与上尿路平滑肌局部肌条收缩有密切关系。Objective To investigate the distribution of interstitial cells of Cajal （ICCs） -the pacemaker ceils of the gut in the different parts of guinea pig pyeloureteral tract and explore the effect on the contraction of smooth muscle. Methods The ureteropelvic junction, upper ureteral, middle ureteral, lower ureteral tissues harvested from 10 guinea pigs were performed KIT immunofluoreseent staining in stretched preparations. Smooth muscle strips of 2 mm × 6 mm were dissected from the sites for tone determination. The frequency and amplitude of the smooth muscle were compared among different sites of pyeloureteral tract. Results Ceils with positive staining by KIT and with similar morphology to ICCs were found in the entire pyeloureteral tract of guinea pigs. The density of ICCs-like cells in the ureteropelvie junction, upper ureteral, middle ureteral, and lower ureteral tissues were （5.20 ＋0.98）, （3.90 ＋0.98）, （3.03 ＋0.98） and （2.50 ＋0.81 ） respectively. The contraction of strips in different sites varied according to the distribution of ICCs. The amplitude and frequency of strips from the ureteropelvie junction and upper ureteral were significantly higher than those from middle ureteral and lower ureteral （P 〈 0. 05 ）. The contraction amplitude and frequency of strips were positively correlated with the nmnber of ICCs （P 〈 0. 05 ）. Conclusion ICCs is unevenly distributed in the upper ureteral tract of guinea pig. The contraction amplitude and frequency of smooth muscle strips are correlated with the number of ICCs in different of sites of pyeloureteral tract.

关 键 词：CAJAL间质细胞 C-KIT受体 上尿路 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R334.2[医药卫生—基础医学]