不同倍性不结球白菜Pol CMS及保持系生理生化特性比较  

Comparison on Physiological and Biochemical Properties of Pol CMS Lines and Their Maintainer Lines in Different Ploid Materials of Non-Heading Chinese Cabbage

作　　者：汤伟华[1,2] 张蜀宁[1] 孔艳娥[1] 张丽丽[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,南京210095 [2]丽水职业技术学院,浙江丽水323000

出　　处：《西北植物学报》2009年第1期80-84,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：江苏省自然科学基金项目(BK2008341)

摘　　要：对二、四倍体不结球白菜Pol CMS及其保持系花蕾和薹叶进行生理生化特性比较分析,结果表明:不育系花蕾中可溶性蛋白、可溶性糖、脯氨酸含量均显著低于保持系,且各指标在不育系中四倍体低于二倍体,而在保持系中则四倍体高于二倍体;不育系MDA含量,POD、SOD、CAT活性均高于保持系,不育系中四倍体均高于二倍体,而保持系中四倍体均低于二倍体;薹叶中MDA含量,POD、SOD、CAT活性均为不育系高于保持系,不育系中四倍体高于二倍体;POD、EST同工酶显示不育系与保持系间均具特异酶带,但不同倍性间并无差异。A comparative study on physiological and biochemical characteristics in the buds and flowering leaves were fulfilled among diploid and autotetraploid Pol CMS lines and their maintainer lines in non-heading Chinese cabbage. The results showed that the contents of solulale sugar,soluble protein and proline in buds of Pol CMS lines were significantly lower than those in their maintainer lines, among which the con- tents in autotetraploid Pol CMS line were lower than those in diploid, while contents in autotetraploid maintainer line were higher than those in diploid; The content of MDA, the activities of POD, SOD and CAT of Pol CMS lines were higher than those in their maintainer lines,among which in autotetraploid Pol CMS line were higher than those in diploid, while in autotetraploid maintainer line were lower than those in diploid;In flowering leaf, the contents of MDA, the activities of POD, SOD and CAT in Pol CMS lines were all higher than those in their maintainer lines, among which in autotetraploid Pol CMS line were higher than those in diploid Pol CMS line. There were obviously differences in POD and EST profile of isozyme analysis between Pol CMS lines and their maintainer lines, but there was no difference between the diploid and autotetraploid materials.

关 键 词：不结球白菜 Pol胞质雄性不育 同源四倍体 生理生化特征 同工酶分析 

分 类 号：Q945.6[生物学—植物学]