水通道蛋白1与胸腔积液发生机制的体内研究  

Expression of Aquaporin-1 in the Pleura in Rats and the Role of Aquaporin-1 in the Pleural Fluid Turnover

作　　者：廖槐[1] 谢灿茂[1] 

机构地区：[1]中山大学附属第一医院呼吸内科,广东广州510080

出　　处：《中国呼吸与危重监护杂志》2009年第2期151-155,共5页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：广东省自然科学基金(编号:5300762);广东省科技计划项目(编号:2005B30601017)

摘　　要：目的探讨水通道蛋白1(AQP1)在胸腔积液发生机制中的作用。方法SD大鼠按实验设计分为5组(n=24):地塞米松组、内毒素组、红霉素组、高渗盐水组及对照组,通过免疫组织化学染色测定AQP1在胸膜上的表达和定位;采用Real-time RT-PCR方法对不同刺激因子作用下不同时间胸膜组织AQP1的基因表达进行定量分析。结果大鼠脏、壁层胸膜间皮细胞及壁层胸膜下毛细血管内皮上均存在AQP1的表达,脏层胸膜间皮细胞上AQP1的表达较壁层胸膜明显[(7.43±2.02)×104copy/μg比(4.14±1.12)×104copy/μg,P<0.05]。地塞米松、高渗盐水上调大鼠胸膜组织AQP1的表达(P<0.05);红霉素、内毒素下调胸膜组织上AQP1的表达(P<0.05)。结论地塞米松、内毒素、红霉素和高渗盐水刺激下胸膜组织AQP1表达的改变,提示存在炎症因子和渗透梯度下调控AQP1的途径,胸膜组织上AQP1可能参与了炎症性胸腔积液的分泌和吸收。Objective To investigate the expression of aquaporin-1 （AQP1 ） in visceral and parietal pleura in SD rats and to examine the effect of AQP1 on pleural fluid turnover. Methods Five groups （ n = 24） of SD rats were randomly assigned to received intrapleural injection of dexamethasone, lipopolysaccharide, erythromycin, hypertonic saline and normal saline, respectively. The AQP1 protein in pleural was detected with immunohistochemistry. The mRNA expression of AQP1 under stimulations at different time points was measured by real time RT-PCR. Results AQP1 was immunolocalized predominantly to the microvessels and mesothelial cells of visceral and parietal pleura. The extent of AQP1 expression in parietal pleura was less than that in visceral pleura[ （4. 14 ± 1.12） ×10^4 copy/ug vs （7.43 ± 2. 02） ×10^4 copy/ug,P 〈0. 05]. AQP1 expression increased at all phases in the dexamethasone group and the hypertonic saline group, whereas decreased in the erythromycin group and the lipopolysaccharide group. Conclusion The stimulations of dexamethasone,lipopolysaceharide,erythromycin and hypertonic saline can significantly change the AQP1 expression in pleura, which indicate that AQP1 may contribute to the accumulation and clearance of pleuritic fluids.

关 键 词：水通道蛋白1 胸膜 胸腔积液 地塞米松 内毒素 红霉素 高渗盐水 

分 类 号：R561[医药卫生—呼吸系统]