旋毛虫成虫抗原和肌幼虫抗原保护性免疫的比较研究  

Comparative study of the protective immunity activated by antigens of adult worms and muscle larvae of Trichinella spiralis

作　　者：毛立群[1] 唐洁[1] 杨静[1] 李覃[1] 牛秀珑[1] 叶路[1] 孙奕[1] 

机构地区：[1]武警医学院免疫学教研室,天津300162

出　　处：《细胞与分子免疫学杂志》2009年第3期208-210,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：武警部队总部级课题(WKH2002-6)

摘　　要：目的:比较旋毛虫成虫抗原和肌幼虫抗原诱导小鼠产生保护性免疫效果的异同。方法:制备旋毛虫成虫抗原和肌幼虫抗原,分组免疫小鼠,并于末次免疫后10d用旋毛虫感染性幼虫对各组小鼠实施攻击感染,检测攻击感染后各组小鼠肠道成虫和肌肉幼虫数并计算减虫率,同时采用ELISA法检测各组小鼠血清IgG抗体水平。结果:成虫抗原免疫组小鼠和肌幼虫抗原免疫组小鼠检获的肠道成虫数和肌幼虫数均显著少于对照组(P<0.05),其中以成虫抗原免疫组小鼠减虫率最高(P<0.01)。成虫抗原免疫组和肌幼虫抗原免疫组小鼠于实施旋毛虫感染性幼虫进行攻击感染的当日所检测的血清IgG抗体水平与其免疫前相比均升高(P<0.01,P<0.05),与对照组相比亦升高(P<0.01,P<0.05),其中也以成虫抗原免疫组小鼠血清IgG抗体升高最为显著(P<0.01)。结论:旋毛虫成虫抗原和肌幼虫抗原均能激发小鼠产生保护性免疫,其中成虫抗原免疫组小鼠显示出更好的抗感染保护性。AIM：To compare the immune protective effects of the mice immunized by antigens derived from adult worms and muscle larvae of Trichinella spiralis.METHODS：The adult worm and muscle larvae antigens of Trichinella spiralis were prepared and two groups of mice（adult worm antigen group and muscle larvae antigen group）were immunized with them,respectively.After 10 days of the final vaccination,the mice in each group were challenged with infective larvae of Trichinella spiralis.The number of intestinal adult worms and muscle larvae were examined and their reduction rate was calculated.Meanwhile,the level of serum IgG in each group was detected by ELISA.RESULTS：Compared with control group,the number of intestinal adult worms and muscle larvae in adult worm group and muscle larvae antigen group was lower（P〈0.05）and number of intestinal adult worms and muscle larvae in adult worm antigen group was at the lowest（P〈0.01）.When the mice in the two groups were challenged with infective larvae of Trichinella spiralis,the titer of serum IgG detected on the same day was higher than that before they were vaccinated with the antigens（P〈0.01,P〈0.05）and it was also higher than that in control group（P〈0.01,P〈0.05）.The level of serum IgG of the mice detected on the day when they were challenged with infective larvae of Trichinella spiralis was significantly higher than that before the mice were vaccinated and it was also higher than that in adult worm antigen group（P〈0.01）.CONCLUSION：Both the adult worm and muscle larvae antigens of Trichinella spiralis can stimulate mice to produce protective immunity and the mice immunized with adult worm antigens have stronger resistance to the subsequent challenge infection.

关 键 词：旋毛虫 成虫抗原 肌幼虫抗原 保护性免疫 

分 类 号：R392.11[医药卫生—免疫学]