流式微球载体技术在检测肾综合征出血热患者血清特异性抗体和细胞因子中的应用  

Application of flow microbeads assay in detection of specific antibodies and cytokines in serum from patients with hemorrhagic fever with renal syndrome

作　　者：张洁[1] 林远[2] 金伯泉[3] 

机构地区：[1]陕西省人民医院中心实验室,陕西西安710068 [2]西安交通大学医学院第一附属医院,陕西西安710061 [3]第四军医大学免疫学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2009年第3期245-247,250,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：陕西省科技厅攻关计划资助项目(2006K09-G11)

摘　　要：目的:建立流式微球载体技术(FMA)检测肾综合征出血热(HFRS)患者血清抗HFRS病毒特异性抗体IgM和IgG及细胞因子含量的新方法。方法:选择28例临床确诊的HFRS患者及20例健康人血清标本,FMA定量检测抗HFRS病毒IgM和IgG;定量检测细胞因子IL-6和TNF-α。检测结果与ELISA法进行比较。结果:FMA检测HFRS患者抗HFRS病毒IgM和IgG的阳性率分别为92.85%和71.43%,健康对照组的抗体阳性率(假阳性率)为0;HFRS患者血清IL-6和TNF-α的含量分别为(532.62±397.19)ng/L和(392.68±177.68)ng/L,明显高于健康对照组(38.77±20.32)ng/L(P<0.01)和(15.91±6.91)ng/L(P<0.01)。ELISA法检测HFRS患者抗HFRS病毒IgM和IgG的阳性率分别为71.43%和50.00%,健康对照组的抗体阳性率(假阳性率)为0;HFRS患者血清IL-6和TNF-α的含量分别为(256.46±102.51)ng/L和(45.63±5.32)ng/L,高于健康对照组(53.8±19.21)ng/L(P<0.01)和(5.81±3.58)ng/L(P<0.01)。结论:建立了FMA法对HFRS患者的特异性抗体和IL-6和TNF-α的检测,其灵敏度明显优于ELISA法,为HFRS的临床诊断和病理机制研究提供了新的方法。AIM：To establish a flow microbeads assay（FMA）to examine the level of hemorrhagic fever with renal syndrome virus（HFRS V.）specific IgM,IgG and cytokines in HFRS patients.METHODS：Serum samples from 28 cases of HFRS and 20 healthy controls were studied.Serum levels of anti-HFRS V.antibodies were qualified and inflammatory cytokines IL-6 and TNF-α were quantified by FMA.The results were compared with the results by ELISA.RESULTS：FMA showed that the positivity rates for anti-HFRS V.IgM and IgG were 92.85% and 71.43%,respectively.None was detected positive in healthy control group.The serum level of IL-6 and TNF-α in HFRS group were（532.62±397.19）ng/L and（392.68±177.68）ng/L,respectively,which were significantly higher than that in healthy control group（38.77±20.32 ng/L and 15.91±6.91 ng/L,P〈0.01）.In contrast,ELISA showed that the positivity rates for anti-HFRS V.IgM and IgG were 71.43% and 50.00%,respectively.None was detected positive in healthy control group.The serum level of IL-6 and TNF-α in HFRS group were（256.46±102.51）ng/L ang（45.63±5.32）ng/L,respectively,which were significantly higher than that in healthy control group（53.80±19.21 ng/L and 5.81±3.58 ng/L,P〈0.01）.CONCLUSION：A FMA method is established to monitor the immune response in HFRS patients,which has better sensitivity than ELISA,and thus provides a useful tool for study of HFRS.

关 键 词：肾综合征出血热 IL-6 TNF-α 流式细胞术 免疫酶技术 

分 类 号：R392.33[医药卫生—免疫学]