含川芎嗪的玻璃化液对异体神经移植免疫反应的影响  

Early effects of vitreous cryopreservation with tetramethylpyrazine on immunological response of nerve allograft

作　　者：袁新[1] 蒋电明[1] 陈增刚[1] 刘建文[1] 黄英如[1] 欧方生[1] 

机构地区：[1]重庆医科大学附属第一医院骨科,重庆400016

出　　处：《重庆医科大学学报》2009年第2期190-193,共4页Journal of Chongqing Medical University

摘　　要：目的:观察含不同浓度川芎嗪(Tetramethylpyrazine,TMP)的玻璃化保存液对异体神经移植的免疫学变化,以探索含川芎嗪的玻璃化液保存同种异系(体)神经后对其抗原性的影响。方法:在Wistar大鼠的背部和腹部皮下植入经过含川芎嗪的玻璃化液保存的SD大鼠的坐骨神经,对照组植入新鲜自体神经。用免疫组化染色检测异体神经中T淋巴细胞的侵润程度。结果:含320mg/L川芎嗪的玻璃化液在-20℃保存异体神经3周后,CD4+和CD8+淋巴细胞的侵润程度明显轻于含其他浓度川芎嗪的玻璃化液保存的异体神经移植组,神经组织较好保持原形态,周围仅见极少量淋巴细胞侵润,与自体神经植入组无明显差别。结论:含320mg/L川芎嗪的玻璃化液在-20℃保存同种异体神经3周后的抗原性明显降低,植入体内后免疫排斥反应不明显。Objective： To observe the change of immunity in vivo after Vitreous cryopreservation with tetramethylpyrazine nerve allograft was implanted into the recipient so as to judge its immunogenicity. Methods ： Sciatic nerves of Sprague-Dawley rats were resected and made by Vitreous cryopreservation with tetramethylpyrazin.The nerves from Sprague-Dawley rats were implanted into the dorsal and ventral subcutis of Wistar rats in experimental group o Fresh nerves from Sprague-Dawley rats were implanted into the dorsal and ventral subcutis of Sprague-Dawley rats in control group respectively.T lymphoeytes infiltraion in isograft nerve was investigated by immunohistochemical Methods： staining CD4 and CD8 positive T lymphocytes with anti-CD4 and CD8 monoclonal antibodies（MoAb）. Results：320 mg/L tetramethylpyrazine combined vitreous cryopreservation stainings at-20℃ for 3 weeks showed that the number of CD4 and CD8 positive T lymphocytes infiltrated into the nerve implants of the experimental group was much lower than that of other tetramethylpyrazine combined vitreous cryopreservation in the control group.there was no marked difference of the 320mg/L tetramethylpyrazine combined vitreous cryopreservation stainings at -20℃for 3 weeks nerves group compared with autografting group. Conclusion：The nerve treated by 320 mg/L tetramethylpyrazine combined vitreous cryopreservation at -20℃ for 3 weeks shoued lower immunogenicity after implantation, and there was no significant rejection response after transplantation in rats.

关 键 词：移植 同种 移植免疫学 T淋巴细胞 

分 类 号：R651.3[医药卫生—外科学] R285.5[医药卫生—临床医学]