改良超声波萃取-高效液相色谱法分离、检测川楝树皮提取物中川楝素  

Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation

作　　者：刘小玲[1] 颜伟伟[1] 王进[1] 张彦[1] 易钢[1] 何於娟[1] 

机构地区：[1]重庆医科大学临床检验诊断学教育部重点实验室,重庆400016

出　　处：《重庆医科大学学报》2009年第2期203-205,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金项目(No:30171150)

摘　　要：目的:建立改良的快速分离、检测川楝树皮提取物中川楝素(Toosendanin,TSN)的超声波萃取-高效液相色谱法。方法:采用超声波乙醇提取-氯仿萃取制备川楝树皮提取物,高效液相色谱法(High performance liquid chromatography,HPLC)分析提取物中的TSN含量,以乙腈-水(45∶55)为流动相,检测波长215nm,流速1ml/min,柱温:25℃,色谱柱为XDBC18柱(4.6×250mm,5μm)。结果:TSN在4.64～88.2μg/ml浓度范围内有良好的线性关系(r=0.9996),测定方法平均加标回收率为100.83%,精密度试验RSD为1.42%,稳定性试验RSD为2.26%。提取物中TSN经HPLC分离提纯后纯度达96.32%。结论:本方法简便、快速、准确,所得结果稳定、重复性好,可作为实验室少量制备和鉴定TSN纯品的方法。Objective： To establish an improved transonic extraction-high performance liquid chromatography （HPLC） method for separating and detecting toosendanin （TSN） in Melia toosendanin extractive. Methods： Transonic alcohol-chloroform extraction method was used to extract TSN from the bark of Melia toosendanin sieb.et zuccc. The content determination of TSN was performed on an Agilent XDB C18 Column（4.6 ×250mm,5μm）. Elution was performed using 45% acetonitrilewater solution as the mobile phase at alflow rate of lml/min. Detection was performed at 215 nm wavelength. Results： The linear regression equation for peak area-TSN concentration （4.64 × 88.2 μg/ml） was： A = 7.616 2C-2.567 7, r=-0.999 6; mean recovery of standard substance was 100.83 % ;RSD of precision test was 1.42%; RSD of stability test was 2.26%. Conclusion： The method is simple, rapid, stable and roducible, which can be used as a method for preparation and identification TSN of Melia toosendanin extractive in laboratory.

关 键 词：川楝素 超声波萃取 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]