复方烫疡油促进深Ⅱ°烫伤创面愈合、减少瘢痕形成的实验研究  

The experimental study of effects of fu fang tang yang you on healing and reducing the proliferation scars of deep partial thinkness burn in rats

作　　者：罗聪[1] 安洪[2] 刘传康[1] 李明[1] 范伟[2] 杨正钦[3] 杨洲[4] 

机构地区：[1]重庆医科大学附属儿童医院骨科,重庆400016 [2]重庆医科大学附属第一医院骨科,重庆400016 [3]重庆医科大学药理学教研室,重庆400016 [4]川北医学院附院普外科,南充637100

出　　处：《重庆医科大学学报》2009年第2期238-242,共5页Journal of Chongqing Medical University

基　　金：重庆大学研究生教育创新基金(2004A028)

摘　　要：目的:观察复方烫疡油(Fu fang tang yang you,FTY)促进小鼠创面愈合、减少瘢痕形成的作用,并探讨其作用机制。方法:采用小鼠深Ⅱ0烫伤模型。创面外用复方烫疡油治疗,并用表皮生长因子(Recombinant human epidermal growth factor,rhEGF)、磺胺嘧啶银霜、芝麻油(赋形剂)作疗效对比,用生理盐水作空白对照。测定创面组织创伤后8、12、24、48、72h干湿重比例以了解组织创伤后水肿情况;透明膜描绘-求积议测定创面伤后3、7、14h创面愈合情况;并用免疫组织化学法观察Ⅰ、Ⅲ型胶原、TGF-β1(Transforming growth factor-β1,TGF-β1)的变化;TUNEL法定量观察组织细胞的凋亡指数;原位杂交观察胶原酶(Marix Metalloproteianse1,MMP-1)及金属蛋白酶抑制剂(Tissue inhibitor of metalloproteinase-1,TIMP-1)mRNA的表达。结果:复方烫疡油能减轻创面炎症,降低创伤组织水肿,改善Ⅰ、Ⅲ型胶原比例,减少瘢痕形成,促进创面愈合。结论:复方烫疡油具有抗炎、抗感染,促进创面愈合,减轻瘢痕形成的作用。Objective：To investigate the effects and the therapeutic mechanism of Fu Fang Tang Yang of You （FTY） on wound healing and reducing the proliferation scar of deep partial thinkness nurn in rats. Method 120 kun-ming rats were employed as the model. Deep partial thinkness burn wounds（3 cm2 per wound ） were made on the back of each rat by 108℃ steam. The wound rats were divided into five groups. Four groups underwent（groupA, B, C, D ） local treatment with FTY, rhEGF, Sulfadiazine silver cream and Vegetable oil, respectively. The control group E was localy treatment with applied the normal saline. Each wound area was calculated by dry/wet weight at 8,24,48,72 h after injury. Histological examination： Determination of TGF- β 1, type of collagen Ⅰ,Ⅲ with immunohistochemistry. Expression of MMP-1, TIMP-1 were testd by in situ hybridiz-ation and image analgsis at 3,7, 14 past-burn days. The apoptosis index was exmined by TUNEL technique. Results：Treatment with FTY could protect the remains of cells and tissues in wound, alleviate inflammatory response, decrease the level of tissues edema, reduce the formation of proliferation scars and accelerate the process of wound healing. Conclusion. FTY could promote the wound healing of deep partial thinkness burn with less scar formation.

关 键 词：烧伤 愈合 瘢痕 复方烫疡油 

分 类 号：R644[医药卫生—外科学]