抗凝溶栓后大鼠腔静脉内皮扫描电镜变化  

Scanning electron microscopic changes of inferior venous wall after anticoagulation and thrombolysis in rats

作　　者：杨冰[1] 赵庆华[1] 赵渝[2] 代远斌[2] 王学虎[2] 向东洲[2] 

机构地区：[1]重庆医科大学附属第一医院护理部,重庆400016 [2]重庆医科大学附属第一医院血管外科,重庆400016

出　　处：《重庆医科大学学报》2009年第2期243-245,共3页Journal of Chongqing Medical University

摘　　要：目的:观察肝素、尿激酶及肝素加尿激酶治疗急性大鼠下腔深静脉血栓(Deep vein thrombosis,DVT)形成后不同时相腔静脉内皮扫描电镜变化。方法:SD大鼠135只,105只建立大鼠急性下腔静脉血栓模型后随机分为肝素A组、尿激酶B组、肝素加尿激酶C组各35只;另30只为假手术组。各组给药后分别于血栓形成后第1、4、7、14、28d获取病变段血管,通过扫描电镜观察静脉内皮层变化。结果:7d时扫描电镜结果显示B及C组内皮损伤较轻,内皮损伤分级主要为Ⅱ～Ⅲ级,而C组为Ⅲ～Ⅳ级(P<0.05);28d时C组内皮损伤较A、B组轻(P<0.05),内皮损伤分级主要为Ⅱ级,而B、C组为Ⅲ～Ⅳ级(P<0.05)。结论:肝素联合尿激酶治疗大鼠急性下腔静脉血栓形成较其单独使用有更好的内皮结构保护作用。Objective： To compare the scanning electron microscopic changes of venous endothelium after acute inferior vein thrombosis in rats. Methods： The inferior caval venous thrombosis models were treated with heparin （n = 35, group A）, urokinase （n =35, group B）, and combination of heparin and urokinase （n =35, group C）, respectively. Sham group （n =30）was also established. The thrombosed caval veins were taken 1,4,7,14, and 28 days after thrombosis. Scanning electron microscopy was used to observe the damage of endothelial cells. Results： The Results showed that the endotheliurn injury degree was mainly Ⅱ to Ⅲgrade in group B and C whereas it was Ⅲ to Ⅳ grade in group A on day7 （P〈0.05）; endotheliurn injury degree was mainly Ⅱ grade in group C whereas it was Ⅲho IVgrade in group A and B on day 28 （P〈0.05）. Conclusion： Combination of heparin and urokinase for the treatment of acute inferior caval venous thrombosis has good short or intermediate term effect on the protection of endothelial structure.

关 键 词：静脉血栓形成 抗凝溶栓 血管内皮 

分 类 号：R654.4[医药卫生—外科学]