TNF-α抑制剂对重症急性胰腺炎大鼠肾损伤的影响  

Influence of tumor necrosis factor-α inhibitor in rats with SAP-associated kidney injury

作　　者：汤志刚[1] 纪涛[1] 邱陆军[1] 黄强[1] 王成[1] 李建生[1] 许戈良[1] 

机构地区：[1]安徽医科大学附属省立医院普通外科,安徽合肥230001

出　　处：《中国普通外科杂志》2009年第3期220-223,共4页China Journal of General Surgery

基　　金：安徽省卫生厅基金项目(05A004)

摘　　要：目的探讨肿瘤坏死因子α(TNF-α)抑制剂对重症急性胰腺炎(SAP)大鼠肾损伤的影响。方法60只SD大鼠随机分成假手术组,SAP组,TNF-α抑制剂治疗组,每组20只。各组建模48 h后处死动物并收集标本,测血清淀粉酶,TNF-α,尿素氮和肌酐;应用TUNEL法检测肾细胞凋亡,计算凋亡指数(AI);并用免疫组化法检测肾脏组织NF-κB的表达,RT-PCR检测ET-1 mRNA的表达。结果假手术组的各项指标均低于SAP组和治疗组(P<0.05)。SAP组TNF-α,血清淀粉酶,尿素氮,肌酐和NF-κB IOD水平分别为(185.36±10.95)ng/L,(7 257.30±361.20)U/L,(17.28±0.87)mmol/L,(78.83±3.02)μmol/L和316.25±20.90,治疗组以上指标分别为(124.32±15.11)ng/L,(6182.60±291.63)U/L,(13.66±0.88)mmol/L,(68.68±3.38)μmol/L和241.90±19.04,两组间比较差异有统计学意义(P<0.05)。SAP组AI为3.33±0.49,明显低于治疗组的7.04±0.41(P<0.05)。SAP组中ET-1 mRNA水平明显高于治疗组和假手术组(P<0.05)。结论TNF-α抑制剂可促进大鼠肾脏细胞凋亡,减轻炎症反应对肾的损伤,改善肾脏功能。Objective To investigate the influence of tumor necrosis factor-α inhibitor on kidney injury in rats with severe acute pancreatitis （ SAP ）. Methods 60 SD rats were randomly divided into 3 groups （ n = 20 in each group ）, including sham group, SAP group, and TNF-α I treatment group. After the models established, the rats were killed after 48h. The levels of serum amylase, tumor necrosis factor-α, urea nitrogen and creatinine were detected. Apoptotic cells were detected by TUNEL. Nuclear-κB was detected by immunohistochemical method. The expression of ET-1 mRNA was detected by RT-PCR. Results All of the indicators in sham group were significantly lower than those in treatment group and SAP group （ P 〈 0.05 ）. The levels of tumor necrosis factor-a, serum amylase, urea nitrogen, creatinine and nuclear-κB IOD were （185. 36 ±10. 95 ） ng/L, （7 257.30 ±361.20）U/L,（17.28 ±0.87）mmol/L,（78.83 ±3.02） μmol/L, 316.25 ±20.90 in the SAP group and （124.32±18.11）ng/L,（6182.60±291.63）U/L, 1 （ 13.66 ± 0.88 ） mmol/L, （ 68.68 ± 3.38 ） tLm01/L, 241.90 ± 19.04 in treatment group, the difference was statistical between the 2 groups （ P 〈 0. 05 ） , while the apoptotic index in the SAP group （ 3.33 ± 0.49 ） was markedly lower than that in the treatment group （ 7.04 ± 0.41 ） （ P 〈 0.05 ）. The expression of ET-1 mRNA in the SAP group was higher than that in the treatment group and sham proup （ P 〈 0. 05 ）. Conclusions The tumor necrosis factor-α inhibitor can promote the cell apoptosis of kidney, ameliorate the kidney injury caused by inflammation, and improve kidney function.

关 键 词：胰腺炎 急性坏死性 肿瘤坏死因子-α 内皮素-1 核因子-ΚB 细胞凋亡 

分 类 号：R657.51[医药卫生—外科学]