新型若丹宁催化荧光法测定痕量钴  

Determination of Trace Cobalt by Catalytic Kinetic Spectrofluorimetry With Novel Rhodanine

作　　者：程晓亮[1] 葛慎光[1] 戴平[1] 张丛丛[1] 于京华[1] 

机构地区：[1]济南大学化学化工学院,山东济南250022

出　　处：《济南大学学报（自然科学版）》2009年第2期149-151,共3页Journal of University of Jinan(Science and Technology)

基　　金：山东省自然科学基金(Y2007B07);山东省重点学科基金(XTD0705);国家十一五支撑计划子课题(2006BAJ03A09-06)

摘　　要：研究新合成的3-对氟苯基-5-（2＇-胂酸基苯偶氮）若丹宁（4FRAAP）在邻苯二甲酸氢钾-氢氧化钠介质中与过氧化氢的反应，发现痕量钴（Ⅱ）能强烈催化该反应的发生，建立了催化动力学荧光法测定痕量钴（Ⅱ）的新方法。研究结果表明，pH=5．0的邻苯二甲酸氢钾-氢氧化钠缓冲溶液，在λ_ex/λ_em=307nm／407nm处，反应体系的荧光值与钴（Ⅱ）的质量浓度在0～12μg·L^-1范围内呈线性关系，检出限达6．71×10^-6g·L^-1。该方法用于猪肝中钴的测定，获得了较为满意的结果。Catalytic kinetic fluoremetry method is developed for the determination of trace amount of cobalt, based on its intensively catalytic effect on the oxidation of the new reagent of 3 - p - fluorophenyl - 5 - o - arsenoxylphenylazo rhodanine by hydrogen peroxide under the buffer solution of potassium hydrogen phthalate - sodium hydroxide. The result showed that the intensity of fluorescence is proportional ,under the experimental conditions of potassium hydrogen phthalate -sodium hydroxide of pH = 5.0 and λ_ex/λ_em = 307 nm/ 407 nm,to the concentration of cobalt in the range of 0 - 12 μg·L^-1 ,and that the detection limit is 6.71×10^-6 g·L^-1. This method has been applied to determine trace cobalt in pigs＇ liver with satisfied result.

关 键 词：3-对氟苯基-5-(2’-胂酸基苯偶氮)若丹宁 催化荧光法 钴(Ⅱ) 

分 类 号：O657[理学—分析化学]