川芎嗪干预长春新碱抑制骨髓基质细胞增殖的研究  

Study of Ligustrazine Hydrochloride Promotes the Proliferation of Bone Marrow Stromal Cells Inhibited by Vincristine

作　　者：文珠[1] 胡国柱[2] 俞火[1] 戎吉平[1] 

机构地区：[1]江西省医学科学研究所血液学研究室 [2]江西省人民医院临床医学研究所,南昌330006

出　　处：《实用临床医学（江西）》2009年第1期1-3,共3页Practical Clinical Medicine

摘　　要：目的探讨川芎嗪(LH)促进小鼠骨髓基质细胞(BMSC)生长及干预长春新碱(VCR)抑制小鼠BMSC增殖的作用。方法①LH5、10、20、40μg/mL,以及VCR2.5、5、10、15μg/mL分别与BMC共同培养14d,测定细胞增殖。②LH5、10、20、40μg/mL与BMC预培养1h后再分别加入终浓度为5μg/mLVCR继续培养14d,测定细胞增殖。结果LH20、40μg/mL组、VCR10、15μg/mL组OD值与对照组比较差异均有统计学意义(均P<0.05),LH20、40μg/mL组OD值与LH10μg/mL组比较差异均有统计学意义(均P<0.05);VCR2.5μg/mL组OD值与VCR15μg/mL组比较差异有统计学意义(P<0.05)。LH10μg/mL+VCR5μg/mL组、LH20μg/mL+VCR5μg/mL组、LH40μg/mL+VCR5μg/mL组OD值与VCR5μg/mL组比较差异均有统计学意义(均P<0.05);LH20μg/mL+VCR5μg/mL组、LH40μg/mL+VCR5μg/mL组OD值与对照组比较差异均有统计学意义(均P<0.05)。结论VCR明显抑制BMSC生长;LH可促进BMSC生长且具有干预VCR对BMSC增殖的抑制作用。Objective To explore the effect of ligustrazine hydrochloride（LH） for injection on the proliferation of bone marrow stromal cells（BMSC） inhibited mice by vineristine（VCR）. Methods ①The marrow cells were cultured with LH （5,10,20,40 μg/mL） and VCR （2.5,5,10. 15 μg/mL） in 37 ℃ 5%CO2 for 14 days, respectively and their effect on marrow stromal cells proliferation was determined. ②The marrow cells were pretreated by LH（5.10.20,40 μg/mL） for 1 h,then cultured with VCR 5 μg/mL for 14 days,and the effect of LH on marrow stromal cells proliferation was determined. Results There was statistical signi tween LH groups of 20.40μg/mL,VCR groups of 10.15 ug /mL and ficance for OD value becontrol group（P〈0. 05, respectively）. There was statistical significance for OD value between LH groups of 20.40μg/mL and LH group of 10 μg/mL （P〈0.05, respectively）. There was statistical significance for OD value between VCR group of 2.5 μg/mL and VCR group of 15μg/mL（P〈0.05）. There was statistical significance for OD value between groups of LH 10 ug /mL^-1 VCR 5 μg/mL, LH 20 μg/mL＋VCR 5 μg/mL, LH 40 μg/mL＋VCR 5μg/mL and group of VCR 5 μg/mL（all P〈0.05）. There was statistical significance for OD value between groups of LH 20 μg/mL＋VCR 5 μg/mL, LH 40 μg/mL＋VCR 5 μg/mL and control group （P〈0.05, respectively）. Conclusion The results suggest that vincristine could inhibit the BMSC proliferation. Ligustrazine could promote the BMSC growth and the proliferation of BMSC inhibited by vincristine.

关 键 词：川芎嗪 长春新碱 骨髓基质细胞 增殖 动物 实验 小鼠 

分 类 号：R282.72[医药卫生—中药学] R329.28[医药卫生—中医学]