咖啡短体线虫分子鉴定  

Molecular identification for Pratylenchus coffea.

作　　者：陈晓玲[1] 陈永红[1] 张洪玲 武目涛[3] 钟国强[3] 方羽生[4] 

机构地区：[1]高明出入境检验检疫局,佛山528500 [2]番禺出入境检验检疫局 [3]广东出入境检验检疫局 [4]华南农业大学

出　　处：《植物检疫》2009年第2期7-9,共3页Plant Quarantine

基　　金：广东省科技厅项目2007B020709006

摘　　要：本实验采用单条线虫法提取DNA,rDNA-ITS-PCR技术扩增目的片段(ITS1、5.8S、ITS2和部分的18S和28S),将此片段纯化回收,克隆测序分析,与GenBank比对,结果为:该种群的基因序列与AY561436(基因库编号)的相似度为92.3%,与AB053485的相似度为91.3%。分子鉴定结果与形态鉴定该线虫为咖啡短体线虫。Single nematode involved the so - called worm lysis buffer was applied to extract the genomic DNA. The product of 1100bp was gained by rDNA - ITS - PCR, which was identical with expected aim fragment. The fragment was purified and reclaimed . The fragment was cloned and sequenced. The sequenced results sligned with nucleotide array in GenBank. The aligned resuhs showed GUDO had 92.3% and 91.3% similarity with array code AY561436 and AB053485 respectively.

关 键 词：咖啡短体线虫 单条线虫 rDNA—ITS—PCR分子鉴定 

分 类 号：S435.712[农业科学—农业昆虫与害虫防治] S41-30[农业科学—植物保护]