TiO_2纳米粒子共振光散射法测定血清白蛋白  

Determination of Human Serum Albumin by Resonance Light Scattering Method with TiO_2 Nanoparticles

作　　者：梁丽娜[1] 葛广华[1] 董存智[1] 

机构地区：[1]淮北煤炭师范学院化学与材料科学学院,安徽淮北235000

出　　处：《淮北煤炭师范学院学报（自然科学版）》2009年第1期60-63,共4页Journal of Huaibei Coal Industry Teachers College(Natural Science edition)

基　　金：安徽省教育厅自然科学基金资助项目(2006KJ127B)

摘　　要：以六偏磷酸钠(SHMP)为修饰剂,制备TiO2纳米粒子.研究TiO2纳米粒子与人血清白蛋白(HSA)的相互作用,建立共振光散射法测定微量白蛋白的新方法.在最佳实验条件下,该方法的线性范围是0.2～65.0mg/L,检出限为0.168mg/L.此方法用于人血清样品中白蛋白的测定,回收率为99.3%～100.6%.The TiO2 nanoparticles were prepared and modified with sodium hexametaphosphate. The interaction of TiO2 nanoparticles with human serum albumin （HSA） is studied. A new resonates light scattering （RLS） method for determination of albumin has been developed. Under the optimal conditions, linear relationships was found between the enhanced intensity of RLS and the concentration of HSA in the range from 0.2 mg/L to 65.0 mg/L. The detection limit was 0. 168 mg/L. The method has been applied to the determination of albumin in human serum samples, and the recovery rate was 99.3 % to 100.6 %.

关 键 词：TIO2纳米粒子 人血清白蛋白 共振光散射 

分 类 号：O657.3[理学—分析化学]