鸡奇异变形杆菌亚单位疫苗的研制及其生物学特性测定  

Manufacture and Bionomics determine of Subunit Vaccine in Avian Proteus mirabilis

作　　者：王红雷 郭文龙[2] 

机构地区：[1]济南市长清区牧政执法大队,山东250300 [2]山东农业大学动物科技学院,泰安271018

出　　处：《家禽科学》2009年第3期12-14,共3页Poultry Science

摘　　要：采用超声波裂解和超速离心法提取鸡奇异变形杆菌中的外膜蛋白作为抗原,制备油乳剂型(Oil Emulsion OE)亚单位疫苗。该亚单位疫苗为油包水型(W/O),稳定性良好,安全可靠。对3周龄雏鸡用该亚单位疫苗肌注接种,6周龄攻毒。试验结果表明,接种800μg OMP/只油乳剂型亚单位疫苗的雏鸡对奇异变形杆菌V3、V4株的攻击具有显著的免疫保护力。Ultrasonic dispersion and ultracentrifugation were used to extract Proteus mirabilis outer membrane protein,its content was determined as antigen and prepared oil emulsion subunit vaccine.The vaccine belongs to water-in-oil microemulsion with better stability, safety and reliability.3-week chicken were vaccinated by i.m. and challenged six weeks later.Result showed that the best immunizing does is 800μg outer membrane protein respectively,at this time the chickens vaccinated subunit vaccine will have good immunogenicity against Proteus mirabilis V3 and V4.

关 键 词：奇异变形杆菌 亚单位疫苗 生物学特性 外膜蛋白 

分 类 号：S852.5[农业科学—基础兽医学]