华支睾吸虫第二中间宿主胞囊蚴的发现与实验观察  

Discovery and Experiment of Clonorchis sinensis Encysted Metacercariae in Second Intermediate Host

作　　者：程荣联 张仁平[1] 杨焰[1] 曹勇[1] 喻珊[1] 秦雪松[1] 陈太梅 李家猷 

机构地区：[1]重庆市涪陵区疾病预防控制中心,重庆涪陵408000

出　　处：《寄生虫病与感染性疾病》2009年第1期1-5,共5页Parasitoses and Infectious Diseases

摘　　要：目的证实麦穗鱼体内存在一个胞囊内有2个以上不同成熟度华支睾吸虫囊蚴的个体(简称"胞囊蚴")。方法计算机检索中文期刊全文数据库、中国生物医学文献数据库、重庆维普数据库和中国优秀博士学位论文数据库,手工检索《中国人体寄生虫病文献提要》,同时检索纳入文献的参考文献。用麦穗鱼和其他鱼类华支睾吸虫囊蚴感染资料,分析麦穗鱼对华支睾吸虫感染的亲和力与适应性;现场调查和从疫区采集麦穗鱼隔离饲养,观察胞囊蚴感染状况。直接压片镜下摄制胞囊蚴照片。用含有胞囊蚴的鱼肉感染粪检阴性的猫,30d后解剖收集成虫。结果纳入分析的54篇文献,麦穗鱼感染率高于其他鱼类的有50篇,占92.59%,同一水域麦穗鱼、其他鱼类囊蚴平均感染率分别为48.15%、13.52%(P<0.001),现患OR和OR95%C.I.分别为5.94和5.66～6.23;囊蚴每尾最高为166个～17042个。现场调查、隔离饲养麦穗鱼胞囊蚴感染率分别为43.70%(274/627)、28.03%(44/157)。感染3只猫获虫率为27.16%(110/405)。结论有5项证据证实麦穗鱼体内,有一个胞囊内存在2个以上不同成熟度华支睾吸虫囊蚴的胞囊蚴感染。Objective To verify that there exist more than 2 Clonorchis sinensis metacercariae （encysted metacercariae） with different maturity degree in one cyst. Method Four electronic databases （ CNKI, CBM, VIP and Chinese Excellent Doctoral Thesis）, the literature summary of Chinese parasitic diseases, along with the reference list cited by included articles were searched. The affinity and adaptation of the infection of Pseudorasbora parva for Clonorchis sinensis were analysed by comparing the data of metacercariae infection status between Pseudorasbora parva and other fishes. The encysted metacercariae infective situation was observed by field investigation and isolatedly feeding P. parva collected from the epidemic areas. The photographs of encysted metacercariae were taken through direct compression under microscopes. The fish meat containing encysted metacercariae was used to infect healthy cats, and 30 days later, the cats＇ were dissected and the adult worms collected. Result Totally 54 articles were included, and of which, 92. 59% （50 articles） reported that the infection rate of P. parva was higher than that of other fishes. In the same water area, the average metacercariae infection rates of P. parva and other fishes were respectively 48.15% and 13.52% （P 〈 0. 001 ）. Prevalence OR （95% C. I. ） was 5.94 （5.66 - 6.23 ）. The estimated maximum amount of metacercariae was 166 - 17 042 per fish. The encysted metacercariae infection rates of P. parva from field investigation and isolated feeding were 43.70% （274/627） and 28.03% （44/157）, respectively. The rate of gaining worms from cats infected by the fish meat including encysted metacercariae was 27.16% （ 110/405 ）. Conclusion It is evident that there exist more than 2 Clonorchis sinensis metacercariae with different maturity degree in one cyst of Pseudorasbora parva.

关 键 词：华支睾吸虫 第二中间宿主 胞囊蚴 囊蚴 

分 类 号：R383.22[医药卫生—医学寄生虫学]