智利竹筴鱼3群体遗传关系初步研究  

Study on genetic relationship of three populations of chilean jack mackerel,Trachurus murphyi from the Southern Pacific

作　　者：张敏[1] 许永久[2] 王成辉[3] 谢峰 

机构地区：[1]上海海洋大学海洋科学学院,上海201306 [2]中国水产科学研究院东海水产研究所,上海200090 [3]上海海洋大学水产与生命学院,上海201306 [4]上海开创远洋渔业有限公司,上海200082

出　　处：《上海海洋大学学报》2009年第2期212-217,共6页Journal of Shanghai Ocean University

基　　金：2006年农业部公海渔业资源探捕调查项目“东南太平洋(秘鲁外海)竹鱼资源探捕”;上海市捕捞学重点学科建设项目(T1101);大洋生物资源开发和利用上海市高校重点实验室资助项目

摘　　要：2006-2007年,对东南太平洋智利外海智利经济区内、外采集的3批智利竹筴鱼群体的线粒体DNA细胞色素b基因(Cytb)的部分序列进行了测定,对遗传关系进行了初步分析。在39个测序样本发现11个单倍型,其中一个为3个群体的共享单倍型。在730 bp的分析序列长度中,共有26个变异位点,其中2006、2007年经济外群体分别有3、22个变异位点,2006年经济区内群体没有变异位点;它们的单倍型多样性指数分别为0.330 9、0.640 5、0.000 0;核苷酸多样性指数分别为0.000 5、0.004 3、0.000 0。AMOVA分析表明:3个群体间不存在显著的遗传分化。邻接法(NJ)构建的系统关系树显示,经济区外与经济区内群体的所有个体聚成一个簇群,即经济区内、外的智利竹鱼来自于同一个种群。Mitochondrial cytb gene sequences with 730 bp were analyzed from chilean jack mackerel （ Trachurus murphyi） of the Southeast Pacific collected in Chile out-economic zone in 2006 （ OEZ2006）, outeconomic zone in 2007 （OEZ2007） and inter-economic zone in 2006 （IEZ2006）. 11 haplotypes in 39 analyzed samples were observed and only one haplotype was shared among three populations with 23 polymorphic sites in analyzed 730 bp sequence. 3, and 22 polymorphic sites were detected in OEZ2006 and OEZ2007, respectively, and no sequence variation was found in IEZ2006. The haplotype diversities were 0. 330 9, 0. 640 5,0. 000 0, and nucleotide diversities were 0. 000 5, 0. 004 3, 0. 000 0 in OEZ2006, OEZ2007, IEZ2006, respectively. AMOVA analysis indicated that no significant genetic divergence among three populahions. NJ tree also showed that all individuals from three populations clustered into one clade. The results indicated that Chilean Jack mackerel in inter- and out-economic zone in Chile would be one population.

关 键 词：智利竹[竹夹]鱼 经济区 线粒体cyt B基因 东南太平洋 

分 类 号：S917[农业科学—水产科学]