高效液相色谱法测定人血浆中卡铂浓度  

HPLC determination of carboplatin concentration in human plasma

作　　者：谢志红[1] 叶丽卡[1] 周龙书[1] 唐波[1] 

机构地区：[1]广州医学院第二附属医院,广东广州510260

出　　处：《中国医院药学杂志》2009年第6期457-459,共3页Chinese Journal of Hospital Pharmacy

基　　金：广东省医学科研基金资助项目(编号:A2006305)

摘　　要：目的:建立用高效液相色谱法测定人血浆中卡铂浓度的方法。方法:采用Ultimate C18色谱柱(4.5mm×250mm,5μm);流动相为乙腈-0.02mol.L-1磷酸二氢钾溶液(0.5:99.5);流速1.0mL.min-1;检测波长230nm。结果:卡铂在0.77～49.07mg.L-1范围内线性关系良好(r=0.998),最低检测质量浓度为0.77mg.L-1,卡铂低、中、高浓度检测的日内、日间RSD分别为1.32%、4.25%、3.13%和2.77%、4.75%、3.30%。结论:该法具有操作简便可靠,准确,稳定性高等特点,适合应用于卡铂的治疗药物监测和药动学研究。OBJECTIVE To develop an HPLC method for the determination of Carboplatin concentratioin in human plasma. METHODS The Ultimate C18 column （4. 5 mm× 250 mm, 5 μm） was used with acetonitrile -0. 02 mol· L^-1 KH2 PO4 （0. 5 ： 99. 5） as mobile phase. Flow rate was 1.0 mL·min ^-1 and the detective wavelength was 230 nm. RESULTS The calihration curve of carboplatin was linear over the range of 0. 77 - 49. 07 mg·L^-1 （r= 0. 998）. The detection limit was 0. 77 mg·L^-1. Theintra day and inter-day RSD were 1.32% ,4. 25% ,3. 13% and 2.77% ,4.75% ,3.30%. CONCLUSION This method is simple, precise, stabile and suitable to be applied in therapeutical drug monitoring of carboplatin and its pharmacokinetics studiy.

关 键 词：卡铂 血药浓度 高效液相色谱法 

分 类 号：R969[医药卫生—药理学]