大黄和泽泻提取物对二甘醇致小鼠肾脏损伤的保护作用研究  

Protective Effect of Extracts from Radix et Rhizoma Rhei and Alisma orientalis against Acute Kidney Injury Induced by Diethylene Glycol

作　　者：朱深银[1] 周远大[1] 杜冠华[2] 

机构地区：[1]重庆医科大学附属第一医院药剂科,重庆市400016 [2]中国协和医科大学/中国医学科学院药物研究所,北京市100050

出　　处：《中国药房》2009年第9期641-643,共3页China Pharmacy

基　　金：国家高技术研究发展计划(863计划)重大专项项目(2004AA2Z3782)

摘　　要：目的:研究大黄和泽泻提取物对二甘醇(DEG)致小鼠肾脏损伤的保护作用及其机制。方法:采用ig给予DEG方法建立小鼠肾脏损伤模型,ig给予大黄和泽泻提取物后测定血清中肌酐(Cr)和尿素氮(BUN)浓度,测定超氧化物歧化酶(SOD)和谷胱甘肽过氧化酶(GSH-PX)活性及丙二醛(MDA)含量变化。结果:小鼠ig给予DEG后有明显中毒表现,肾脏体质量比增加,血清中Cr和BUN水平升高,肾组织SOD和GSH-PX活性降低、MDA含量增加;给予大黄和泽泻提取物后小鼠中毒表现明显减轻,肾脏体质量比减少,血清中Cr和BUN水平明显降低,肾组织SOD和GSH-PX活性升高、MDA含量降低。结论:大黄和泽泻提取物对DEG所致小鼠肾脏损伤具有明显的保护作用,该作用与其改善肾脏抗氧化酶活性及抑制脂质过氧化反应有关。OBJECTIVE： To explore the protective effect of the extracts from Radix et Rhizoma Rhei and Alisma orientalis against acute kidney injury induced by diethylene glycol （DEG） and its action mechanism. METHODS： The acute kidney injury model of mice was induced by intragastrie administration of DEG. Then the model mice were administered intragastrically with the extracts of Radix et Rhizoma Rhei and Alisrna orientalis. Serum levels of creatinine（Cr） and blood urea nitrogen （BUN）, the activities of superoxide dismutase （SOD） and glutathione peroxidase（GSH-PX） and the content of malonaldehyde（MDA） were determined. RESULTS： After administration of DEG, the mice presents with obvious toxic reactions including the increase of the ratio of kidney to body weight, the increase of serum levels of BUN and Cr, the decrease of the activities of SOD and GSH-PX in kidney tissue, the increase of the content of MDA. However, after treatment with the extracts of Radix et Rhizoma Rhei and Alisma orientalis, the toxic symptoms in mice were attenuated markedly, the ratio of kidney to body weight decreased, serum levels of BUN and Cr decreased significantly, the activities of SOD and GSHPX in renal tissues increased and the content of MDA decreased. CONCLUSION： The remarkable protective effect of the extracts from Radix et Rhizoma Rhei and Alisrna orientalis against DEG-induced acute kidney injury in mice might be related to its actions of improving the activity of antioxidase of kidney while inhibiting lipid peroxidation.

关 键 词：二甘醇 肾脏损伤 大黄提取物 泽泻提取物 脂质过氧化 

分 类 号：R285.5[医药卫生—中药学] R965.1[医药卫生—中医学]