HPLC法同时测定元胡止痛片中3种活性成分的含量  

Determination of Three Active Components in Yuanhu Zhitong Tablet By HPLC

作　　者：王光发[1] 廖正根[1] 梁新丽[1] 赵国巍[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌市330004

出　　处：《中国药房》2009年第9期672-674,共3页China Pharmacy

基　　金：国家科技支撑计划子课题(2006BAI09B07-05);江西省自然科学基金资助项目(2007GZY0932)

摘　　要：目的:建立以高效液相色谱法同时测定元胡止痛片中延胡索乙素、欧前胡素和异欧前胡素含量的方法。方法:色谱柱为Zorbax Extend-C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(三乙胺调pH6.5,梯度洗脱),流速为1mL·min-1,柱温为25℃。结果:延胡索乙素、欧前胡素、异欧前胡素的检测浓度分别在10.2～51.0、2.5～12.5、2.2～11.0μg·mL-1(均r=0.9999)范围内与各自峰面积积分值呈良好的线性关系;三者加样回收率分别为97.89%、101.77%、99.04%,RSD分别为0.8%、1.4%、1.3%(均n=9)。结论:本方法简便、快速、准确,可用于元胡止痛片的质量控制。OBJECTIVE： To develop an HPLC method for simultaneous determination of tetrahydropalmatine, imperatorin and isoimperatorin in Yuanhu zhitong tablet. METHODS： The sample was separated on A Zorbax Extend - C18 column （250 mm ×4.6 mm, 5 μm） at column temperature of 25℃ . The mobile phase consisted of acetonitrile - 0.1% phosphoric acid solution （adjusted pH to 6.5 by triethylamine, gradient elution） at a flow rate of 1 mL·min^-1. RESULTS： The linear ranges of tetrahydropalmatine, imperatorin and isoimperatorin were 10.2--51.0μg ·mL^-1（ r - 0.999 9）, 2.5- 12.5μg·mL^-1（ r = 0.999 9） and 2.2-11.0μg·mL^-1（ r = 0.999 9） respectively, and their average recovery rates were 97.89% （RSD = 0.8%, n = 9）, 101.77% （RSD = 1.4%, n = 9） and 99.04% （RSD - 1.3%, n = 9）, respectively. CONCLUSION： The method is simple, rapid, accurate, and applicable for the quality control of Yuanhu zhitong tablet.

关 键 词：元胡止痛片 延胡索乙素 欧前胡素 异欧前胡素 高效液相色谱法 含量测定 

分 类 号：R283.64[医药卫生—中药学] R927.2[医药卫生—中医学]