HPLC法测定雪隆胶囊中东莨菪素和伞形花内酯的含量  

HPLC analysis of scopoletin and umbelliferone in Xuelong capsules

作　　者：杨燕霞 白杨[2] 刘西京[3,4] 贾强[3,4] 

机构地区：[1]广东省城市建设高级技工学校,广东广州510650 [2]中科院广州生物医药与健康研究院,广东广州510275 [3]深圳清华大学研究院,广东深圳518057 [4]深圳市创新中药及天然药物研究重点实验室,广东深圳518057

出　　处：《广东药学院学报》2009年第1期37-39,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：深圳市科技局资助项目(深圳科信局[2007]334)

摘　　要：目的测定雪隆胶囊中东莨菪内酯和伞型花内酯的含量。方法采用高效液相色谱法,色谱柱为Phenomenex C18(250 mm×4.6 mm,5μm),流动相为甲醇-四氢呋喃水溶液进行梯度洗脱,检测波长为346 nm。结果东莨菪内酯平均回收率为99.3%,RSD为0.81%,伞型花内酯的平均回收率为99.6%,RSD为1.03%。结论本法简便、准确、精密度高、重现性好,可用于该制剂的质量控制。Objective To establish an HPLC method for analyzing the contents of scopoletin and umbelliferone in Xuelong capsules. Methods Samples were analyzed on a Phenomenex Cls column （250 mm×4.6 mm, 5 μm） using methanol-tetrahydrofuran-water as mobile phase. The detection wavelength was at 346 nm. Results The average recovery of scopoletin was 99.3% with RSD of 0.81%. The average recovery of skimmetin was 99.6% with RSD of 1.03%. Conclusion The method is simple, accurate and reproducible for the quality control of Xuelong capsules.

关 键 词：雪隆胶囊 东莨菪内酯 伞形花内酯 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]