疏肝健脾方药对非酒精性脂肪性肝病大鼠肝细胞PI3K p85α蛋白表达的影响  

Effects of soothing liver and invigorating spleen recipes of TCM on expression of PI3K p85α protein in hepatocyte of rats with non-alcoholic fatty liver disease

作　　者：杨钦河[1] 欧健[1] 孙升云[2] 乔娜丽[1] 程少冰[1] 陈万群[1] 金玲[2] 杨环文[1] 李娜[1] 纪桂元[1] 谢维宁[1] 林秀峰[1] 张玉佩[1] 薛川松[1] 

机构地区：[1]暨南大学医学院,广东广州510632 [2]暨南大学附属第一医院,广东广州510632

出　　处：《广东药学院学报》2009年第1期62-67,共6页Academic Journal of Guangdong College of Pharmacy

基　　金：国家中医药管理局科研专项基金(02-03JP39);中国博士后科学基金(2002031295);广东省中医药局科研课题(2050041)

摘　　要：目的观察疏肝健脾方药对非酒精性脂肪性肝病(NAFLD)大鼠肝细胞磷脂酰肌醇-3-激酶p85α(PI3K p85α)表达的影响。方法以高脂饮食12周建立大鼠NAFLD模型后,造模组大鼠再随机分为模型组、疏肝组、健脾组、综合组和自然恢复组等5组。治疗组分别给予相应药物灌胃,其他组给予等量蒸馏水灌胃,模型组继续高脂饮食,其余均予基础饲料喂养。治疗8周后,用3%戊巴比妥腹腔麻醉,腹主动脉取血,全自动生化仪检测血脂及肝功,稳态模型法评价胰岛素抵抗程度,光镜下观察各组肝脏病理改变,免疫组化方法检测肝细胞内PI3K p85α蛋白的表达情况。结果与模型组相比,药物治疗各组及自然恢复组肝脏脂变程度明显减轻,肝功、血脂、胰岛素抵抗均有显著改善(P<0.05或P<0.01),肝细胞内PI3K p85α蛋白的表达明显减少(P<0.05)。与自然恢复组相比,药物治疗各组肝功、血脂、血糖的改善无显著差异,但肝脏脂变程度减轻,胰岛素抵抗指数明显下降(P<0.05),肝细胞内PI3K p85α蛋白的表达显著降低(P<0.05)。结论疏肝健脾方药对高脂饮食诱导的大鼠NAFLD有较好的治疗作用,其机制可能是与其降低肝细胞PI3K p85α的表达有关。Objective To observe the effect of soothing liver or invigorating spleen recipes of Traditional Chinese Medicine （TCM） on expression of PI3K p85α protein in hepatocyte of rats with non-alcoholic fatty liver disease（ NAFLD）, and to explore their mechanisms of it. Methods The rat models of NAFLD were established by feeding on high fat diet for 12 weeks. Then the model rats were randomly subdivided into 5 subgroups： model group, soothing liver group, invigorating spleen group, integrated group and natural recovery group. Rats in medication groups were fed with corresponding herbs by gastric perfusion while the remaining rats were fed by equal distilled water. Meantime, rats in model group were continuously given high fat diet while all other rats were given normal diet. 8 weeks later, all rats were anesthetized by intraperitoneal injection of 3% pentobarbital. ALT and AST contents in the serum and together with blood lipid were detected by automatic biochemical analyzer. The degree of insulin resistant was measured by homeostasis model assessment. Hepatic tissue was stained to undergo pathohistology observation. The expression of PI3K p85α protein in hepatocyte was measured by immunohistochemistry. Results Compared with model group, the hepatic steatosis degree were lighten obviously in medication groups and natural recovery group, the levels of ALT, AST, blood lipid and insulin resistance were ameliorated significantly （ respectively, P 〈 0.01 ; P 〈 0. 05 ） , expressions of PI3K p85α protein in hepatocyte were reduced significantly （ P 〈 0. 05 ）. Compared with the natural recovery group, the levels of ALT, AST, blood lipid in medication groups were shown no statistical significance, but hepatic steatosis degree were lighten, insulin resistance index and expressions of PI3K p85α protein in hepatoeyte were reduced significantly（ P 〈 0.05 ）. Conclusion Soothing liver, invigorating spleen methods and recipes showed certain therapeutic effect on rats with NAFLD, which may be due to its

关 键 词：非酒精性脂肪性肝病 胰岛素抵抗 PI3Kp85α 疏肝健脾方药 

分 类 号：R285.5[医药卫生—中药学]