扶正解毒含药血清对染硫化镍人支气管上皮细胞膜脂质过氧化的影响  

Effects of Fuzhengjiedu-containing Serum on the Lipid Peroxidation of Bronchus Epithelium Cell Membrane of Nickel Sulfide Exposed Workers

作　　者：王婷[1] 朱玉真[1] 吴瑞贤 谢玉芳 殷苏雅 黄克英 刘应凤 帅丽 

机构地区：[1]兰州大学中西医结合研究所,甘肃兰州730000 [2]金川有色金属公司职工医院,甘肃金昌737100

出　　处：《工业卫生与职业病》2009年第2期81-83,共3页Industrial Health and Occupational Diseases

基　　金：教育部博士点专项科研基金(20050730031)

摘　　要：目的探讨扶正解毒含药血清(FJD)拮抗硫化镍(NiS)所致细胞毒作用及其作用机制。方法采用体外细胞培养方法,观察FJD对染NiS人支气管上皮(16HBE)细胞膜脂质过氧化产物丙二醛(MDA)含量,超氧化物歧化酶(SOD)、活性氧(ROS)、谷胱甘肽过氧化物酶(GSH-Px)活力的影响。结果NiS能明显增加MDA含量及ROS活力,与阴性对照组比较,差异有统计学意义(P<0.01);NiS能明显降低SOD及GSH-Px活力,与阴性对照组比较,差异有统计学意义(P<0.01)。在体外染NiS 16HBE细胞培养过程中加入不同浓度的FJD(低、中、高)剂量,中、高剂量组可提高16HBE细胞内SOD及GSH-Px活力(P<0.05),具有剂量-效应关系,FJD能显著抑制MDA生成量及ROS活力,以中、高剂量为甚(P<0.05)。结论NiS可增强细胞脂质过氧化,FJD具有拮抗NiS所致细胞毒性的作用,可能与其抗氧化功能有关。Objective To study the antagonizing effect of fuzhengjiedu-containing serum on cell toxicity induced by nickel sulfide（NiS）exposure and its mechanism. Methods The bronchus epithelium cells（16HBE） were cultured in vitro. The effects of fuzhengjiedu-containing serum on lipid peroxidation products of NiS exposed cells including MDA content, activity of SOD, GSH Px, and reactive oxygen species（ROS）were observed. Results NiS could increase the of MDA and ROS activity, the difference was significant compared with the control group（P〈0.01）, meanwhile, NiS could decrease the expression of SOD and GSH-Px, the difference was significant compared with the control group（P〈0. 01）. During the incubation of NiS exposed 16HBE in vitro, the addition of medium and high dosages of fuzhengjiedu-containing serum could increase GSH-Px and activity of SOD showing dose-dependent relationship. The fuzhengjiedu-containing serum at medium and high dosages could distinctively inhibit the MDA content produced and ROS activity（P〈0.05）. Conclusion NiS might increase lipid peroxidation of the cells. Fuzhengjiedu-containing serum might antagonize the NiS induced cell toxicity which might associate with its anti-oxidation function.

关 键 词：硫化镍 扶正解毒含药血清 丙二醛 超氧化物歧化酶 活性氧 谷胱甘肽过氧化物酶 

分 类 号：R285.5[医药卫生—中药学]