胰腺腺泡细胞胞浆游离钙浓度的测定及药物作用的研究  

FLUORESCENCE MEASUREMENT OF CYTOSOLIC FREE Ca^(2+) CONCENTRATION IN PANCREATIC ACINAR CELLS AND STUDY OF DRUG'S ACTION

作　　者：陈学群[1] 杨文修[1] 胡文卫 李俊英[1] 许文胜[1] 王津 余奕 

机构地区：[1]南开大学物理系,天津300071

出　　处：《南开大学学报（自然科学版）》1998年第1期51-55,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：本文建立了用荧光指示利Fura－2测定胰腺腺泡细胞胞浆游离Ca2＋浓度（［Ca2＋］i）的实验技术，包括：胰腺腺泡的分离制备；Fura—2／AM的负载；腺泡悬液的荧光光谱测量等．测定了胰腺腺泡细胞［Ca2＋］i。的静息值和卡巴可促腺泡细胞［Ca2＋］i升高的剂量—效应关系及动力学过程，并研究了柴胡总皂甙对胰腺腺泡细胞［Ca2＋i］的影响．发现0．01mg／ml浓度的柴胡总皂甙使胰腺腺泡细胞［Ca2＋］。明显升高，0．1mg／ml浓度时可使［Ca2＋］。升高到静息值的3倍，提示柴胡总皂甙的促酶分泌作用是通过胞内［Ca2＋］i升高介导的．A technique for measurement of cytosolic free Ca2+ concentration [Ca2+]i in pancreatic acinar cells is eatablished, including preparation of dispersed pancreatic acini, Fura - 2 loading, measurement of relative fluorescence of pancreatic acini suspension. Resting cytosolic free Ca2+ concentration and effect of carbachol on rising [Ca2+]i is measured. The effect of saikosaponin on [Ca2+ ]i in pancreatic acinar cells is studied. It is found that [Ca2+]i rises with saikosaponin concentration increasing, 100μg/mL saikosaponin raises [Ca2+]i three-fold from resting value. These results suggest that [Ca2+ ]i rising mediates the stimulatory effect of saikosaponin on enzyme secretion from pancreatlc acini.

关 键 词：胰腺腺泡 柴胡总皂甙 细胞胞浆 钙离子 

分 类 号：R285.5[医药卫生—中药学] Q26[医药卫生—中医学]