反义PARP基因真核表达重组体的构建及其HeLa转化细胞系的建立  

CONSTRUCTION OF ANTISENSE PARP cDNA RECOMBINANTS AND ESTABLISHMENT OF THE HeLa TRANSFORMANT AFTER ANTISENSE PLASMID INTRODUCING

作　　者：杨其伟[1] 史国利[1] 陈雅文[1] 刘丛[1] 陈德风[1] 

机构地区：[1]南开大学分子生物学研究所,天津300071

出　　处：《南开大学学报（自然科学版）》1998年第1期59-63,112,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金!39480016

摘　　要：利用DNA体外重组技术将PARP基因cDNA部分序列反向克隆到真核表达载体pMAMneo上，构建成重组质粒pMAMneo－C1．4和pMAMneo－C0．3．将重组质粒pMAM－neo－C1．4转染HeLa细胞，经G418筛选，建成细胞系HeLa－C1．4－neo，Southern杂交结果表明，外源PARP基因cDNA部分序列已稳定整合到受体细胞基因组中，该细胞系的建立为研究聚ADP核糖基化作用在细胞内的功能打下了基础．The recombinants pMAMneo-C1. 4 and pMAMneo-C0. 3 were constructed by inserting varied partial length cDNAs of poly (ADP-ribose) polymerase gene into mammalian expressive vector pMAMneo respectively. The transformed cell line, HeLa-C1. 4-neo, capable of generating antisense RNA for poly (ADP-ribose) polymerase gene was obtained by introducing pMAMneo-C1. 4 plasmid into HeLa cells. Southern blot analysis showed that the exogenous partial cDNA for poly (ADP-ribose) polymerase had been integrated into the cell chromosomes. The established cell line laid a foundation for evaluating the biological roles of poly (ADP-ribosyl) action in eukaryotic cells.

关 键 词：PARP基因 DNA转染 Hela转化细胞系 真核表达 

分 类 号：Q78[生物学—分子生物学]