诱导根瘤菌与玉米结瘤共生的研究  

Studies on inducing nodulation in maize by Rhizobium

作　　者：潘建菁[1,2] 陈启锋[1,2] 黄世贞[1,2] 

机构地区：[1]福建农业大学作物科学学院 [2]福建省农业科学院土壤肥料研究所

出　　处：《福建农业大学学报》1998年第1期10-15,共6页Journal of Fujian Agricultural University

摘　　要：以２，４－Ｄ处理玉米苗并接种根瘤菌，诱导根瘤菌与玉米苗结瘤共生．试验结果如下：５个玉米品种的始瘤期仅需２～３ｄ，结瘤率高达９０％～１００％，固氮酶活性（乙炔还原法）在４５～５５ｎｍｏｌ株－１ｄ－１之间．在闽单８８中，还出现琼脂液化、结红瘤等特殊现象．经显微镜观察表明，根瘤菌可从弯曲、膨大、变形的根毛侵入，可形成类侵入线；瘤组织细胞内含有数量众多的根瘤菌菌体．说明了２，４－Ｄ能诱导根瘤菌进入非豆科植物根瘤，并能在瘤内繁殖．Rhizobium was introduced into maize roots for nodulation (para nodules) and symbiosis by the addition of synthetic auxin 2,4 D.The results were following.Among 5 tested maize varieties,the date of the first nodule appearance was only 2 to 3 days later.The nodulation rate was high,all reaching 90%-100%;nitrogenase activity varied from 45 to 55 nmol per plant a day. In Mindan 88,there were some special phenomena such as agar dissolved and red nodule like structures.Observations through microscope proved that Rhizobium could invade into the root along the curling, swolling and warping root hair,which formed the thread like structure for infection. There were numerous rhizobia in the intercellular spaces and cells inside the nodule.All those indicated that rhizobia could be induced into non legume plant roots and propagated inside the nodules.

关 键 词：玉米 根瘤菌 诱导结瘤 共生固氮 

分 类 号：S513.01[农业科学—作物学]