Two genetically distinct units of Sinomanglietia glauca (Magnoliaceae) detected by chloroplast PCR-SSCP  

作　　者：Zhi-Rong ZHANG Lai-Chun LUO Ding WU Zhi-Yong ZHANG 

机构地区：[1]Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China [2]Jingdezhen Comprehensive College, Jingdezhen 333000, China

出　　处：《Journal of Systematics and Evolution》2009年第2期110-114,共5页植物分类学报（英文版）

基　　金：funded by the Foundation from Education Department of Jiangxi Province (2007-149);the National Natural Science Foundation of China (30460020);the Cultivation Programs for Young Scientists of Jiangxi Province (2008DQ01500)

摘　　要：Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those in Jiangxi. Because of the new findings and the poor reproducibility inherent to RAPD and ISSR markers of previous studies, the population structure of this rare species was reanalyzed with chloroplast PCR-SSCP （single-stranded conformation polymorphism）, including all of four recorded populations. The results showed that two distinct haplotypes characterized Jiangxi and Hunan populations separately, with no genetic variation occurring within regions. We postulated that this surprising pattern might result from habitat fragmenta- tion and demographic bottlenecks during and/or after the Quaternary glaciation. On the basis of the pronounced genetic structure, two evolutionarily significant units （ESUs） were recommended for effective conservation of S. glauca.Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those in Jiangxi. Because of the new findings and the poor reproducibility inherent to RAPD and ISSR markers of previous studies, the population structure of this rare species was reanalyzed with chloroplast PCR-SSCP （single-stranded conformation polymorphism）, including all of four recorded populations. The results showed that two distinct haplotypes characterized Jiangxi and Hunan populations separately, with no genetic variation occurring within regions. We postulated that this surprising pattern might result from habitat fragmenta- tion and demographic bottlenecks during and/or after the Quaternary glaciation. On the basis of the pronounced genetic structure, two evolutionarily significant units （ESUs） were recommended for effective conservation of S. glauca.

关 键 词：chloroplast genome genetic structure PCR-SSCP Sinomanglietia glauca. 

分 类 号：Q78[生物学—分子生物学] S512.1[农业科学—作物学]