PiA水稻悬浮细胞系的建立  

Rapid establishment of suspension cell lines in japonica rice

作　　者：张红宇[1,2] 杨富云[1] 高梅[1] 徐培洲[1] 张全芳[1] 徐建第[1] 吴先军[1,2] 

机构地区：[1]四川农业大学水稻研究所,温江611130 [2]四川农业大学西南作物基因资源与遗传改良教育部重点实验室,雅安625014

出　　处：《生物工程学报》2009年第3期424-427,共4页Chinese Journal of Biotechnology

基　　金：教育部长江学者和创新团队发展计划(No.IRTO453)资助~~

摘　　要：本研究以抗稻瘟病的粳稻PiA开花后12～15d的幼胚为材料,将诱导10～15d的愈伤不经固体培养基继代,直接转到AA液体培养基上,在较短时间内成功建立起了优良的水稻悬浮细胞系;并测定了该悬浮细胞系不同时期的生长特性和分化情况,结果表明悬浮培养适宜的继代天数是7～10d;培养30～120d的细胞分化能力和植株再生能力较好,细胞分化率和成苗率分别为57.1%和20%,为进一步利用悬浮细胞进行遗传转化和原生质体分离奠定了基础。With Jingkang No.5 （PiA）, calli of the PiA induced for 10-15 days were transferred into amino acid liquid culture medium, to establish excellent rice suspension cell lines successfully in a relative short time. The growth characteristics and differentiation conditions of suspension cells were measured at different phases. Results revealed that the optimal subculture time was 7-10 days, and the cells cultured for 30-120 days had the best differentiation ability （57.1%） and regeneration ability （20%）. This study is promising in further using the suspension cell for genetic transformation and protoplasm isolation.

关 键 词：水稻 悬浮细胞系 细胞分化 PIA 

分 类 号：S511[农业科学—作物学] TQ464.1[化学工程—制药化工]