传染性腔上囊病病毒与禽腺病毒混合感染的分子鉴定  

Molecular identification of a coinfection of infectious bursal disease virus with fowl adenovirus

作　　者：何秀苗[1] 官丁明[1] 禤金彩[1] 韦平[2] 

机构地区：[1]广西民族大学,广西南宁530006 [2]广西大学,广西南宁530005

出　　处：《中国兽医科学》2009年第3期223-226,共4页Chinese Veterinary Science

基　　金：广西自然科学基金项目(桂科自0728058);广西科技攻关项目(2007A04024);广西民族大学引进人才启动基金项目(0409025)

摘　　要：应用设计的分别针对传染性腔上囊病病毒(IBDV)VP2蛋白基因高变区(vVP2)和Ⅰ群禽腺病毒(FAV)六邻体(hexon)蛋白基因的特异性PCR引物,对临床疑似IBD病例的2只鸡的腔上囊样品和泄殖腔拭子样品分别进行IBDV和FAV的分子检测。结果显示,通过巢式PCR从2份腔上囊样品中均扩增出了471 bp的IBDV vVP2特异性目的片段;SspⅠ和SacⅠ的酶切分析以及序列测定分析证实,该目的片段属超强毒IBDV(very virulent IBDV,vvIBDV)的特征性序列,与常用疫苗株的亲缘关系较远。从2份泄殖腔拭子样品中均扩增出了900 bp的FAV特异性目的片段;序列测定和遗传进化分析表明,该片段在分子水平上属于血清1型FAV(FAV-1)。结果表明,该病例同时感染了vvIBDV和FAV-1。Specific PCR primers for hypervariable region of VP2 gene （vVP2） of infectious bursal disease virus（IBDV） and hexon gene of fowl adenovirus（FAV）were designed respectively to detect the ge- nomes of IBDV and FAV from bursal and cloacal swab samples of two individual birds in a flock in which an outbreak of IBD was clinically suspected. A 471 bp-fragment specific for IBDV vVP2 was amplified by a nested PCR from the two samples,and digestions with endonueleases Ssp I and Sac I and nucleotides sequencing showed that the fragment had the sequence characters of very virulent IBDV（vvIBDV） strains and was far from the conventional vaccine viruses in phylogenetic relationship. A 900 bp-fragment specific for hexon gene of FAV was amplified from the cloacal swabs, and the nucleotide sequencing and homology analysis showed that the 900 bp-fragment was molecularly categorized as FAV serotype 1.The results demonstrated that the chicken flock was co-infected by vvIBDV and FAV-1.

关 键 词：传染性腔上囊病病毒 禽腺病毒 VP2蛋白基因高变区 六邻体蛋白基因 混合感染 

分 类 号：S855.3[农业科学—临床兽医学]