抗水貂肠炎病毒单克隆抗体的制备及其生物学特性鉴定  

Preparation and biological characteristic of monoclonal antibodies against mink enteritis virus

作　　者：王建科[1] 程世鹏[1] 闫喜军[1] 易立[1] 罗国良 柴秀丽[1] 罗彬[1] 吴威 赵传芳 

机构地区：[1]中国农业科学院特产研究所,吉林132109 [2]吉林中特生物技术有限责任公司,吉林132109

出　　处：《中国兽医科学》2009年第3期227-232,共6页Chinese Veterinary Science

基　　金：吉林省科技发展计划项目(20071120,20080213)

摘　　要：将水貂肠炎病毒SMPV-11株纯化后免疫4-6周龄雌性BALB／c小鼠，取其脾细胞与SP2／0骨髓瘤细胞进行融合，经亚克隆和间接ELISA筛选，获得了8株稳定分泌抗水貂肠炎病毒SMPV-11株单克隆抗体的杂交瘤细胞株，分别命名为D3、H5、F5、F4、A9、B7、H11和H8。经过鉴定：其腹水ELISA效价分剐在1：6.4×10^3和1：2.56×10^4之间；且与犬瘟热病毒、阿留中病病毒、犬腺病毒不发生交叉反应；而与水貂肠炎病毒、犬细小病毒和猫泛白细胞减少症病毒呈特异性反应；这8株单克隆抗体均为IgG类型。Female BALB/c mice of four to six week-old female were immunized with the purified mink enteritis virus（MEV SMPV-11）strain, spleen cells were taken to fuse with SP2/0 myeloma cells and hybridoma cells were cloned by using indirect ELISA and limited dilution methods. 8 hybridoma cell lines steadily secreting monoclonal antibodies（McAbs） against MEV were obtained and designated as D3, H5,F5,F4, Ag,B7,H11 and H8,respectively. The ELISA titers of these MeAbs ranged from 1： 6.4×10^3 to 1：2.56×10^4. Specificity test showed that all the McAbs were specific to MEV,canine parvovirus and feline panleukopenia virus, rus. All the McA but did not react bs were identified with canine distemper virus,Aleutian disease virus and canine adenovirus. All the McAbs were identified to be IgG using Mouse Mab Isotyping Test Kit.

关 键 词：水貂肠炎病毒 单克隆抗体 生物学特性 

分 类 号：S855.3[农业科学—临床兽医学] S852.65[农业科学—兽医学]