金丝桃苷对SLT-Ⅱe诱导大鼠肠黏膜微血管内皮细胞分泌功能的影响  

Effects of hyperoside on the secretions of NO,ET-1,P-selectin,sICAM-1 and TNF-α from rat intestinal microvascular endothelial cells induced by SLT-Ⅱe

作　　者：周宏超[1,2] 高立云[2] 范光丽[1] 刘钟杰[3] 穆祥[2] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]北京农学院兽医学(中医药)北京市重点实验室,北京102206 [3]中国农业大学动物医学院,北京100094

出　　处：《中国兽医科学》2009年第3期271-276,共6页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30671543);北京市自然科学基金项目(6021001);北京市属市管高等学校人才强教计划项目(BAHED)

摘　　要：将肠黏膜微血管内皮细胞分为正常对照组、SLT-Ⅱe对照组及1、5、10μg/mL金丝桃苷处理组5组,采用ELISA法测定了培养3、6、9和12 h时细胞上清液中NO、ET-1、P-选凝素、sICAM-1及TNF-α浓度的变化。结果显示,5μg/mL金丝桃苷可下调SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞NO、ET-1的分泌,细胞培养至第12 h时,NO分泌量为(19.04±1.38)μmol/L,ET-1分泌量为(2.54±0.25)pg/mL。10μg/mL金丝桃苷可下调SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞sICAM-1的分泌,第12 h时其浓度为(9.81±1.92)pg/mL。1、5、10μg/mL金丝桃苷不能下调SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞P-选凝素和TNF-α的分泌。表明,金丝桃苷可通过抑制SLT-Ⅱe诱导肠黏膜微血管内皮细胞NO、ET-1、sICAM-1的过量分泌,缓解肠道微循环障碍,减轻炎症反应,达到治疗水肿病的目的。The cultured rat intestinal microvascular endothelial cells（RIMECs） in vitro were divided into control group,SLT-Ⅱe group, test Group 1 （1μg/mL SLT-Ⅱe ＋1μg/mL hyperoside）, test Group 2（1 μg/mL SLT-Ⅱe ＋5μg/mL hyperoside） and test Group 3（1μg/mL SLT-Ⅱe ＋10μg/mL hyperoside）, respectively. The concentration of NO, ET-1, P-selectin, sICAM-1 and TNF-α in cell supernatant were determined using ELISA at hours 3,6,9 and 12 post cultivation, respectively. The results showed that 5μg/mL hyperoside significantly reduced the secretion of NO and ET-1, and 10μg/mL hyperoside signifi-cantly reduced the secretion of sICAM-1. The secretions of NO, ET-1 and sICAM-1 were （19.04±1.38）μmol/L, （2.54±0.25） pg/mL and （9.81±1.92） pg/mL at hour 12 post-cultivation,respectively. The secretions of P-selectin and TNF-α were not reduced by 1μg/mL,5μg/mL and 10μg/mL hyperoside. It was concluded that hyperoside could reduce the secretions of NO, ET-1 and slCAM-1 from rIMECs to relive instestinal microcirculation obstruction,to alleviate the inflammation response and to treat edema disease of swine.

关 键 词：SLT-Ⅱe 大鼠 肠黏膜微血管内皮细胞 NO ET-1 P-选凝素 SICAM-1 金丝桃苷 

分 类 号：S852.21[农业科学—基础兽医学]