镍污染区大气PM2.5对大鼠心血管系统损伤生物标志物的影响  

Acute Impact of Air PM2.5 of Nickel-contaminated Area on Cardiovascular System of Rats

作　　者：罗斌[1] 牛静萍[1] 阮烨[1] 罗小峰[1] 徐佳[1] 张琳[1] 

机构地区：[1]兰州大学公共卫生学院劳动卫生与环境卫生研究所,甘肃兰州730000

出　　处：《环境与健康杂志》2009年第3期209-211,共3页Journal of Environment and Health

摘　　要：目的探讨镍污染区大气细颗粒物(PM2.5)对大鼠心血管系统损伤生物标志物的影响。方法于2007年6—7月和12月,采集镍污染区和对照区的大气PM2.5,进行部分元素分析;并将42只SPF级雄性Wistar大鼠随机分为生理盐水对照组、镍污染区及对照区PM2.5低(1.6mg/kg)、中(8.0mg/kg)和高(40mg/kg)剂量组,每组6只,经气管分别一次性滴注PM2.5悬液和生理盐水,染毒24h后处死大鼠,测定大鼠血清中可溶性血管细胞黏附分子(sVCAM-1)和心肌组织匀浆单核细胞趋化因子(MCP-1)的含量。结果镍污染区大气PM2.5中镍含量约是对照区的50倍。镍污染区组PM2.5染毒后大鼠心脏组织匀浆中MCP-1含量与染毒剂量成正相关,镍污染区各剂量组均显著性高于对照区各剂量组和生理盐水对照组(P﹤0.05)。镍污染区和对照区各剂量组染毒后大鼠血清中sVCAM-1含量均有所升高。镍污染区中、高剂量组与对照区相应剂量组比较,差异有统计学意义(P﹤0.05,P﹤0.01)。结论镍污染区大气PM2.5对大鼠血清sVCAM-1和心肌MCP-1影响较对照区大,其中镍可能起到了关键性作用。Objective To explore the acute effects of nickel in air PM2.5 on the cardiovascular system of rats. Methods The air PM2.5 of nickel-contaminated area and control area were collected and determined for some major metal elements. 42 male Wistar rats were randomly divided into 10 groups, 6 rats in each,the rats were instilled through trachea with saline, PM2.5 suspension of nickel-contaminated area and the control area respectively, at different doses （low, moderate and high dose）. 24 hours later, all the rats were killed for the determination of sVCAM-1 in the blood and MCP-1 in the myocardial tissue. Results The concentration of nickel in the air PM2.5 of nickel-contaminated area was found to be about 50 times higher than that in the control area. A good dose-response relationship was found in MCP-1 determination of the heart tissue in nickel-contaminated groups, and there were significant differences between nickel-contaminated area group and other groups（P 〈 0.05）. As for the sVCAM-1 in the serum, it was significant higher in the nickel-contaminated group and control group than the saline control group （P 〈 0.05）. Among the groups of nickel-contaminated area and control area, significant differences were found between the moderate and high dose groups. Conclusion The air PM2.5 from nickel-contaminated area has an obvious effect on the MCP-1 and sVCAM-1 of rats, the nickel in the air PM2.5 is likely the main contributing factor.

关 键 词：空气污染 颗粒物 镍 可溶性血管细胞黏附分子 单核细胞趋化因子 

分 类 号：R994.6[医药卫生—毒理学]