分光光度计测定尿液中枸橼酸的浓度  

STUDIES OF DETERMINATION OF CITRIC ACID IN URINE BY SPECTROPHOTOMETER

作　　者：王超[1] 余兆雄[1] 蔡勇[1] 廖泽明[1] 

机构地区：[1]荆门市第一人民医院泌尿外科,448000

出　　处：《中国保健营养（临床医学学刊）》2008年第11期60-62,共3页China Health Care Nutrtion

摘　　要：目的建立一种用分光光度计测定尿中枸橼酸浓度的方法，并评价此方法在临床研究中的价值。方法枸橼酸与溴化钾、高锰酸钾在酸性溶液中生成五溴丙酮，经石油醚萃取，继与硫脲（PH9．2）反应生成水溶性黄色复合物，用分光光度计可在450nm波长处测定。结果枸橼酸在7．472—285．7mg／L内，吸收度与浓度呈良好的线性关系。A=0．2275C＋0．01036，r=0．9999，平均回收率为98．46％（n=5），批内变异系数为1．3％（n=20），且与离子交换色谱法有显著的相关性。结论该方法简单易行，快速准确，适用于临床上尿石症患者尿液中枸橼酸浓度的测定，为临床医师制定个体化的尿石预防方案提供依据。Purpose： To establish a method for assay of citric acid in urine by spectrophotometer and evaluate its application in clinic research. Methods： Citric acid, kalium bromatumand kaliumpermanganicumcould action to produce pentabromoacetone in acid solution. The output was extract by petroleum benzene and with sulfocarbamide（PH9.2） to make yellow compound of water solubility. Be determinate by spectrophotometer in 450 nm wavelength. Results： The final results in treated urine were determinated exactly. This methodhad a significant correlation with colorimetric method. All the interassay and between-assay coefficients of variation were less than 5%. The recoveries for the three urine samples were all more than 95%. Conclusion： The method is feasible, fast and accurate. It can be used for calculus ingredient source diagnosis in urinary calculus patients and offer the foundation of individual preventing urinary calculus program for doctor.

关 键 词：枸橼酸盐 尿液 分光光度法 

分 类 号：R446.12[医药卫生—诊断学]