HPLC-ELSD法测定黑乳海参皂苷甲nobiliside A冻干脂质体中药物含量以及包封率  

HPLC-ELSD determination of content and entrapment efficiency of Holothuria nobilis saponins nobiliside A freeze-dried liposome

作　　者：郭丹[1] 熊阳[1,2] 张悦[1] 崔黎丽[1] 孙鹏[1] 陈建明[1] 

机构地区：[1]第二军医大学药学院,上海200433 [2]浙江中医药大学,杭州310053

出　　处：《药物分析杂志》2009年第3期373-377,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：“十一五”国家高技术研究发展计划(863计划)(2006AA090304)

摘　　要：目的:建立HPLC-ELSD法测定黑乳海参皂苷甲nobiliside A冻干脂质体药物含量以及包封率。方法:采用SB-C18柱;流动相为甲醇-0.5%醋酸二元梯度洗脱;检测器为蒸发光散射检测器;采用微柱离心法分离重建后的nobilisideA脂质体和游离药物。结果:nobiliside A在0.120～1.92μg线性关系良好(r=0.9993);方法平均回收率为99.12%(n=9)。结论:本方法简便快速,结果准确可靠,可用于nobiliside A冻干脂质体含量及包封率的测定。Objective：To develop an HPLC-ELSD method for determination of content and entrapment efficiency of nobiliside A freeze-dried liposome.Methods：HPLC-ELSD was adopted in the quantitative analysis with Agilent SB-C18 column and methanol-0.5% HAc by gradient elution of mobile phase with ELSD detector.Microcolumn centrifugation method was used to determine entrapment efficiency of nobiliside A freeze-dried liposome.Result：The linear range for nobiliside A was 0.120-1.92 μg（r=0.9993）.The average recovery was 99.12%（n=9）.Conclusion：The HPLC-ELSD method is simple,accurate and reliable for the determination of the content and the entrapment efficiency of nobiliside A freeze-dried liposome.

关 键 词：HPLC-ELSD nobiliside A冻干脂质体 包封率 微柱离心法 

分 类 号：R917[医药卫生—药物分析学]