炮制对远志中皂苷元类成分的影响  

Research on the senegenin composition in Polygala tenuifolia with different processing drugs method

作　　者：房敏峰[1] 付志玲[1] 王相人[1] 陈焕[1] 赵桂仿[1] 郑晓晖[1] 

机构地区：[1]西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室,西安710069

出　　处：《药物分析杂志》2009年第3期452-457,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：陕西省社会发展基金项目(2008K16-03);陕西省教育厅产业化培育项目(07JC17)

摘　　要：目的:考察17种不同炮制方法对远志中皂苷元组成和含量的影响,探讨古人炮制意图,为远志炮制机理和饮片质量控制提供依据。方法:采用17种不同炮制方法制备远志饮片,经甲醇超声提取,10%盐酸水解,电喷雾质谱(ESI-MS)分析远志酸解产物中3个主要色谱峰的化学结构,高效液相色谱测定远志皂苷元的含量。结果:炮制辅料能显著促进环远志皂苷元的生成;经过不同方法炮制后,远志皂苷元含量有不同程度的变化。结论:炮制对远志中皂苷元的组成和含量有明显影响。Objective：To provide the basis for Polygala tenuifolia processing mechanism and quality control by study the effects of 17 processing methods on the composition and content of senegenin in P.tenuifolia.In the other hand,to explore the processed intention of ancient.Methods：P.tenuifolia decoction pieces were processed by 17 methods,extracted by methanol and ultrasonic and hydrolyzed in 10% hydrochloric acid.The chemical structure of three main peaks of P.tenuifolia were analized by electrospray mass spectrometry（ESI-MS）,and the contents of senegenin were determined by HPLC.Results：The generation of cyclosenegenin can be promoted significantly by different processing excipient;The contens of P.tenuifolia senegenin have been changed in varying degrees by different processing methods.Conclusion：The composition and contents of senegnin in P.tenuifolia have been obviously affected by processing.

关 键 词：远志 炮制 皂苷元 电喷雾质谱 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]