HPLC法比较熊胆粉及猪胆粉、牛胆粉、羊胆粉和鸡胆粉中胆汁酸类成分  

HPLC comparison of bile acids in Fellis Ursi powder,Fellis Suis powder,Calculus Bovis powder,Fellis Caprinus powder and Fellis Galli powder

作　　者：张贇华 刘建忠 彭霞 李忠琼 张雯洁 

机构地区：[1]云南省食品药品检验所,昆明650011 [2]云南省陇川县章凤制药厂,陇川678700 [3]云南省西双版纳州食品药品检验所,景洪666100

出　　处：《药物分析杂志》2009年第3期487-490,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用HPLC法分别测定熊胆粉与猪胆粉、牛胆粉、羊胆粉和鸡胆粉的胆汁酸类成分并进行比较。方法:色谱柱为Alltech Prevail Select C18(250mm×4.6mm,5μm)柱;甲醇-乙腈-0.03mol.L-1磷酸二氢钠溶液(磷酸调pH4.4)为流动相进行梯度洗脱;检测波长为210nm;柱温为40℃。结果:5种胆汁粉所含结合型胆汁酸和水解后胆汁酸的液相色谱图明显不同。结论:5种胆汁粉可采用液相色谱图进行鉴别。Objective：To determinate the components of the bile acids in Fellis Ursi powder and Fellis Suis powder,Calculus Bovis powder,Fellis Caprinus powder,Fellis Galli powder by HPLC respectively and compare their chromatograms from each other.Method：The separation was performed on a Alltech Prevail Select C18column（250 mm×4.6 mm,5 μm）.The mobile phase was composed of methanol-acetonitrile-0.03mol·L-1 sodium dihydrogen phosphate solution with gradient elution.The detection wavelengthwas 210 nm.The column temperature was 40 ℃.Results：The chromatograms of conjugated bile acids and bile acids by hydrolysis in five galls was clearly different.Conclusion：Five galls can be identified by HPLC.

关 键 词：HPLC 胆汁酸 熊胆 猪胆 牛胆 羊胆 鸡胆 

分 类 号：R917[医药卫生—药物分析学]