多壁碳纳米管-聚茜素红膜修饰电极测定人尿中的尿酸  

Determination of uric acid in human urine using multiwalled carbon nanotubes-polymeric alizarin film modified electrode

作　　者：池永明[1] 李将渊[1] 马曾燕[1] 

机构地区：[1]西华师范大学化学化工学院,化学应用与污染控制技术重点实验室,南充637002

出　　处：《药物分析杂志》2009年第3期494-497,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：四川省教育厅重点科研项目(2004A106

摘　　要：目的:制备多壁碳纳米管-聚茜素红膜(MWNT-PAR)修饰电极,测定尿酸的含量。方法:循环伏安法、线性扫描伏安法和差分脉冲伏安法。结果:在pH=0.83的HCl-KCl缓冲溶液中,尿酸在MWNT-PAR修饰电极上的电氧化是两电子两质子的扩散控制的不可逆过程,氧化峰电流与尿酸浓度在5.0×10-6～1.0×10-4mol·L-1范围内呈良好的线性关系,相关系数r=0.9992,检出限为2.0×10-6mol·L-1。结论:该方法可直接测定人尿样品中尿酸,操作简便,灵敏度高。Objective：To make multiwalled carbon nanotubes-polymeric alizarin film modified electrode（MWNT-PAR）and determine uric acid（UA）with it.Methods：Determine the content of UA with cyclic voltammetry（CV）,linear sweep voltammetry（LSV）and differential pulse voltammetry（DPV）.Results：The electrical oxidation of UA in hydrochloric acid-potassium chloride buffer solution at pH= 0.83 is an irreversible process with two protons and two electrons,and controlled by diffusion.Good linearity was found between oxidation peak current and concentration of UA when concentration was in 5.0×10-6-1.0×10-4 mol·L-1,relative coefficient r was 0.9992,and detection limit was 2.0×10-6 mol·L-1.Conclusion：The method is simple and high selective,which can be used for the determination of uric acid in human urine.

关 键 词：尿酸 多壁碳纳米管 聚茜素红 修饰电极 

分 类 号：R917[医药卫生—药物分析学]