顶空毛细管气相色谱法测定重组人心钠肽中残留乙腈  

Determination of residual solvents in atrial natriuretic peptide by head-space capillary gas chromatography

作　　者：易俊波[1] 买制刚[1] 邱岗峰[1] 李凌云[1] 周兆平[1] 林枫[1] 

机构地区：[1]深圳大学生化工程技术研究中心,深圳518057

出　　处：《药物分析杂志》2009年第3期501-503,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：深圳大学科研启动基金资助项目(项目编号:200822)

摘　　要：目的:建立重组人心钠肽中残留溶剂乙腈的气相色谱分离测定方法。方法:采用DB-624弹性石英毛细管色谱柱(30m×0.32mm,1.8μm),载气为氮气,氢火焰离子化检测器FID,进样口温度为250℃;检测器温度为250℃;柱温为40℃保持8min。分流比10∶1,水为溶剂,乙醇为内标物。结果:使用该方法被测物乙腈能得到良好的分离与测定,峰面积与浓度呈良好的线性关系,精密度良好。结论:方法准确可靠,可用于重组人心钠肽药品中有机溶剂残留量的检测。Objective：This paper reports a GC method for determination of acetonitrile in atrial natriuretic peptide by a head-space GC method.Method：The analytical column was DB-624（30 m×0.32 mm,1.8 μm）.The injector temperature was 250 ℃,the detector used was FID at 250 ℃.The column temperature was 40 ℃ holding 8 min,the carrier gas was nitrogen.The solvent was water.Results：The solvent can be completely separated,and the calibration curves of each solvent had good linear relationship within a certain range.Conclusion：This method is simple,rapid and precise,it is suitable for the measurement of residual solvents in atrial natriuretic peptide.

关 键 词：顶空进样法 毛细管气相色谱法 重组人心钠肽 乙腈 

分 类 号：R917[医药卫生—药物分析学]