利用mRNA差异显示技术研究香菇发育相关基因  

Identification of developmentally related genes in Lentinula edodes by mRNA differential expression

作　　者：蔡为明[1] 李南羿[1] 谢鸣[1] 高中山[2] 金群力[1] 

机构地区：[1]浙江省农业科学院园艺研究所,杭州310021 [2]浙江大学农业与生物技术学院园艺系,杭州310029

出　　处：《菌物学报》2009年第2期289-294,共6页Mycosystema

基　　金：浙江省自然科学基金(No.Y305631)

摘　　要：以香菇子实体不分化突变株和对照菌株L98411为材料,利用mRNA差异显示技术研究二者的基因表达差异,共分离到21条差异片段,其中12个分别与泛素、ATP合成酶、锌指蛋白、GTP结合蛋白、延伸因子g1线粒体前体、过氧化物酶、硫酯酶、类TrpB酶、2,4-二烯酰辅酶A还原酶、糖苷水解酶、热激蛋白、疏水蛋白等已知基因有较高的同源性,这表明香菇子实体分化发育是一个复杂的过程,涉及到胞内转运、转录调控、细胞分化、蛋白合成、各种代谢途径中的酶、抗逆反应等诸多途径的协同调控。Differential-display reverse transcription-PCR （DDRT-PCR） was used to investigate the difference of gene expression between tmfruitificative mutant and wild type strain of Lentinula edodes, and 21 cDNA fragments were obtained. Twelve fragments showed high sequence similarity to known gene products, including ubiquitin, ATP synthase, zinc finger family protein, GTP binding protein, elongation factor gl mitochondrial precursor, peroxiredoxin, thioesterase family protein, 2,4-dienoyl-CoA reductase, pyridoxal-phosphate dependent TrpB-like enzyme, glycoside hydrolase family 65, heat shock protein and hydrophobin. The genes indicate that the fruiting body development in L. edodes is a complex process involving several pathways and signal transduction such as intracellular trafficking, transcriptional regulation, cell division, protein synthesis, metabolism, and stress response.

关 键 词：差异显示反转录PCR 突变体 子实体发育 基因表达 

分 类 号：S646.12[农业科学—蔬菜学]