骨髓间充质干细胞移植对急性心梗大鼠能量代谢关键酶的影响  

Transplantation of MSCs and its impact on the energy metabolism enzymes in acute myocardial infarction rat

作　　者：李望[1] 胡盛寿[1] 魏英杰[1] 张浩[1] 候剑锋[1] 叶波[2] 

机构地区：[1]中国医学科学院北京协和医学院阜外心血管病医院卫生部心血管疾病再生医学重点实验室,北京100037 [2]中国医学科学院北京协和医学院肿瘤医院胸外科,北京100021

出　　处：《基础医学与临床》2009年第3期238-241,共4页Basic and Clinical Medicine

基　　金：国家高技术研究发展计划项目(2006AA02Z4B8)

摘　　要：目的探讨骨髓间充质干细胞(MSCs)对急性心梗的大鼠心肌组织中能量代谢关键酶的影响。方法SD大鼠18只,随机分为假手术对照组、急性心梗组及MSCs移植组。应用ELISA检测大鼠左心室组织中己糖激酶(HK)、肉毒碱脂酰转移酶(CACT)、脂酰辅酶A合成酶(ACS)、柠檬酸合成酶(CS)和乳酸脱氢酶(LDH)的含量及活性。结果大鼠心梗后1 d左心室组织中CACT、ACS、CS和LDH的含量下降、HK的含量和活力增加(P<0.05或P<0.01)。在经过MSCs移植后上述酶的水平基本恢复。结论急性心梗后左心室组织能量代谢的关键酶发生变化,MSCs移植对这种能量代谢的异常变化具有一定的改善作用。Objective To investigate the effects of transplantation of MSCs on energy metabolism enzymes after acute myocardial infarction（AMI）. Methods Eighteen SD rats were randomly divided into 3 groups（ n = 6 respectively） ：control group, acute myocardial infarction group and MSCs group. The left ventricular tissue of rats was isolated one day later after left anterior descending artery occlusion for determination of HK, CACT,ACS, CS, LDH by ELISA. Results One day after AMI, the concentration and activity of these enzymes changed obviously （P 〈 0. 05 - P 〈 0.01 ）. One day after MSCs treatment, both concentration and activity of those enzymes returned to normal levels. Conclusion The transplantation of MSCs may improve eadiocyte＇s energetics by regulating the concentration and activity of the enzymes.

关 键 词：骨髓间充质干细胞 移植 心肌梗死 关键酶 

分 类 号：R542.22[医药卫生—心血管疾病]