大鼠支气管重建后肺神经内分泌细胞数量及分布的改变  

Quantity and distribution of pulmonary neuroendocrine cells of rat following bronchoplasty

作　　者：高坤祥[1] 王云杰[1] 张志培[1] 赵正源[1] 闫小龙[1] 邓迎春[1] 韩勇[1] 

机构地区：[1]第四军医大学唐都医院胸腔外科,陕西西安710038

出　　处：《基础医学与临床》2009年第3期242-246,共5页Basic and Clinical Medicine

基　　金：全军医学科研"十一五"计划课题基金(06Q059)

摘　　要：目的探讨左侧支气管重建后肺神经内分泌细胞(PNEC)数量、分布的变化及在气道损伤、创伤修复过程中的作用。方法30只SD大鼠左侧支气管重建后按取样时间随机分为术后1周、1和3月组,每组10只;另设10只大鼠为正常对照组。用Grimelius银染色法观察PNEC数量及分布,免疫组化法检测五羟色胺(5-HT)阳性PNEC、降钙素基因相关肽(CGRP)阳性PNEC的表达。结果术后1周和1月Grimelius银染色见PNEC多位于肺内支气管上皮,其数量大幅度增加(P<0.01),见有多个PNEC聚集形成的神经小体(NEB),至3月其数量接近正常对照组;免疫组化检测术后1周和1月大支气管5-HT、CGRP阳性PNEC和NEB数量大量增加(P<0.01),至3月其数量接近正常对照组;而小支气管及周围细支气管5-HT、CGRP阳性PNEC和NEB与正常对照组无显著差异。结论支气管重建后PNEC在支气管创伤和修复过程中可能起着重要作用。Objective To explore the role of pulmonary neuroendocrine cells （PNECs） in damage and repair of airway by the changing amount, distribution of PNECs after bronchoplasty. Methods Thirty sprague dawley rats were randomly divided into three groups according to sample taking time after left principal bronchoplasty： 1 week, 1 month and 3 months. Another 10 rats without bronchoplasty were used as control. The quantity and distribution of PNECs were examined by Grimelius silver stain. Both serotonin （5-HT） positive PNECs and calcitonin gene-related peptide （CGRP） positive PNECs were examined by imnunohistochemistry. Results PNECs were mainly found in the epithelium of the bronchus and terminal bronchioles. Some PNECs formed into neuroepithlial bodies （NEB）. One wk after bronchoplasty, PNECs were significantly increased, and then gradually return to the normal control after 3 months. Except their location in the larger bronchi, 5-HT positive PNECs and CGRP positive PNECs did the same in number and NEB formed. Conclusion The quantity of PNEC and 5-HT positive PNEC and CGRP positive PNEC were significantly increased after bronchoplasty. PNEC may take part in damage and repairing process of airway.

关 键 词：支气管重建 神经内分泌细胞 神经上皮小体 降钙素基因相关肽 五羟色胺 

分 类 号：R655.3[医药卫生—外科学]