急性热应激与热惊厥幼龄大鼠海马ZnT3 mRNA表达和代谢变化的^1H-MRS波谱研究  

Hippocampal Zinc transporter 3 mRNA expression and metabolic changes defined by ^1H-MRS in weaning rats following heat stress and febrile convulsion

作　　者：倪宏[1] 陈大庆 郑伟良[3] 水泉祥[4] 

机构地区：[1]苏州大学附属儿童医院儿科医学研究所,苏州215003 [2]浙江省温州市苍南县人民医院儿科,325800 [3]浙江大学附属邵逸夫医院放射科,杭州310003 [4]浙江大学附属儿童医院神经内科,杭州310003

出　　处：《中华神经医学杂志》2009年第3期250-253,257,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30470555,30870808）;江苏省自然科学基金青年科技创新人才技术带头人项目（BK2007509）

摘　　要：目的探讨氢质子磁共振波谱分析（^1H-MRS）结合神经病理手段对急性热应激（HS）和热惊厥（FC）后脑神经元代谢和损伤的早期检测价值。方法采用热水浴诱导21d龄大鼠FC模型，应用^1H-MRS检测HS和FC后脑内N-乙酰天门冬氨酸（NAA）、复合胆碱（Cho）、肌酸（Cr）和乳酸（Lac）含量的变化，原位杂交检测海马锌离子转运体3（ZnT3）mRNA表达。结果MRS检测结果显示对照组、HS组与FC组NAA／Cr比值分别为1．50±0．42，1．57±0．50和1．61±0．37，Cho／Cr比值分别为0．93±0．27，1．13±0．17和1．28±0．31，各组间差异均无统计学意义（P〉0．05）；Lac／Cr仅见于FC组，为0．64±0．23。FC组海马齿状回检测到ZnT3 mRNA表达。结论Lac波峰是惊厥性神经元损伤的特征性^1H-MRS变化；ZnT3与海马苔藓纤维再生性发芽密切相关；单次短暂的FC发作也能使脑神经元早期发生不同于HS的显著的神经活性物质表达和物质代谢的变化，对脑神经元造成一定的损伤。Objective To asses the value of proton magnetic resonance spectroscopy （^1H-MRS） combined with neuropathological findings in early detection of metabolic abnormities and damages of the brain neurons following heat stress （HS） and febrile convulsion （FC）. Methods Febrile convulsion models were established in weaning rats （21 days old） by means of hot water bath. ^1H-MRS was performed to measure the changes in N-acetylaspartate （NAA）, choline （cho）, lactate （Lac） and creatine （Cr） contents in the brain tissue following HS or FC, and in situ hybridization was used to detect Zinc transporter 3 （ZnT3） mRNA expression in the hippocampus. Results In the control group, HS group and FC group, the NAA/Cr ratio （1.5±0.42, 1.57±0.59, and 1.61±0.37, respectively） and Cho/Cr ratios showed no significant differences, but a significant increase in Lac/Cr ratio was observed in FC group. ZnT3 3 mRNA expression was detected in the dentate gyrus of the rats following the onset of FC. Conclusions As Lac increase is a putative marker of seizure-induced neuronal damage, and ZnT3 is associated with mossy fiber sprouting in the hippocampus, our results suggest that even a single temporary FC may result in marked changes in neuronal metabolism and cause subtle brain injury.

关 键 词：热性惊厥 磁共振 波谱 锌离子转运体3 

分 类 号：R686[医药卫生—骨科学]