24例输血患者血型不规则抗体分析  

Analysis of Irregular Antibodies in 24 Blood Recipients

作　　者：杭海[1] 

机构地区：[1]山西省临汾市人民医院输血科,山西临汾041000

出　　处：《中国生物制品学杂志》2009年第3期278-280,共3页Chinese Journal of Biologicals

摘　　要：目的对输血患者中血型不规则抗体检出情况进行分析,探讨不规则抗体筛检及抗体鉴定在临床输血中的意义。方法收集2006年11月～2007年9月,在山西省临汾市人民医院院输血治疗的患者标本3115份,应用盐水法、RhD初筛试验凝聚胺法检测不规则抗体,抗体筛检阳性标本进一步以微柱凝胶抗人球蛋白法鉴定,分析抗体的特异性。结果凝聚胺法检出不规则抗体22份,微柱凝胶抗人球蛋白法检出24份,其中抗-E抗体占20.8%,抗-c抗体占25.0%,抗-D抗体占12.5%,抗-P1抗体占12.5%,抗-Jkb抗体占29.2%。阳性标本中,Rh系统占62.5%,其他系统占37.5%。结论输血前对受血者进行常规的抗体筛检试验,对预防溶血性输血反应,提高临床用血的安全性和有效性具有重要意义。Objective To analyze the detection result of irregular antibodies in 24 blood recipients and explore the significance of irregular antibody screening and antibody identification in clinical blood transfusion. Methods From November 2006 to September 2007, the specimens of 3 115 blood recipients in Linfen People＇s Hospital, Shanxi Province, China were collected and detected for irregular antibodies by blood cross matching tests with physiological saline and polybrene as well as RhD primary screening test, and the positive ones were further identified for specificity by microcolumn gel test and antiglobulin method. Results A total of 22 specimens were judged as positive for irregular antibodies by polybrene method, while 24 by microcolumn gel tests. Of the 24 recipients, 20. 8% were positive for anti-E, 25.0% for anti-c, 12. 5% for anti-D, 12. 5% for anti-P; and 29. 2% for anti-Jkt, antibodies. Of the irregular antibodies, 62. 5% were in Rh system, and 37.5% in other systems. Conclusion Routine antibody screening of recipients before blood transfusion is of important significance in preventing hemolytic transfusion reaction as well as improving the safety and effectiveness of clinical application of blood.

关 键 词：血型 不规则抗体 输血反应 

分 类 号：R551.03[医药卫生—血液循环系统疾病] R457.1[医药卫生—内科学]