无菌兔、SPF兔和普通兔脏器系数的比较  

Comparison of Organ Coefficient in Germ Free,SPF and Conventional New Zealand Rabbits

作　　者：李增强[1] 孙淑华[1] 孟金萍[1] 王艳蓉[1] 杨旭[1] 张琨[1] 刘云波[1] 

机构地区：[1]中国医学科学院北京协和医学院实验动物研究所,北京100021

出　　处：《中国比较医学杂志》2009年第1期37-40,共4页Chinese Journal of Comparative Medicine

基　　金：北京市教委无菌兔技术的应用与推广(DWS200723)

摘　　要：目的对普通级新西兰兔进行剖宫产,获得无菌兔,比较无菌兔、SPF兔和普通级兔的脏器系数。方法采取剖宫产手术获得无菌兔,在无菌隔离器内人工哺乳饲养。用电子天平和刻度尺测量无菌兔、SPF兔和普通兔心、肝、脾、肺、肾、肾上腺、胸腺、脑等脏器的脏器重量和长度,计算脏器系数。结果经检测无菌兔符合国家标准;无菌级、SPF级和普通级新西兰兔在左颌下腺、右颌下腺、心脏、肺、胆囊、脾脏、左肾上腺、右肾上腺、子宫+卵巢、左睾丸、胃长、蚓突长度、盲肠长度、盲肠总重、盲肠干重、胃总重、胃干重等17项指标差异显著(P<0.05);脑、肝脏、左肾、右肾、胰脏、右睾丸、胃宽、小肠长度、直肠长度、大肠长度等10项指标差异不显著(P>0.05)。结论不同等级微生物环境对新西兰兔脏器系数有显著影响。Objective To establish a germ free rabbit model by cesarean section. The organ coefficients ingerm free, SPF and conventional rabbits were compared. Methods The germ free leverets were obtained from conventional rabbit by cesarean section, and the young rabbits were fed with artificial milk by hand in germfree isolator. The organ coefficients in germ free, SPF and conventional rabbits were measured using electronic balance. Results The germ free rabbits were in accordance with the quality standard of germ free rabbits. The coefficients of left submaxillary gland, fight submaxiUary gland, heart, iung, cholecyst, spleen, left adrenal gland, fight adrenal gland, womb ＋ ovary, left testis, length of stomach, procussus vermiformis, length of caecum, gross weight of caecum, dry weight of caecum, gross weight of stomach and dry weight of stomach had significant difference in the 3 groups of rabbits （P 〈 0.05 ）. The brain, liver, left kidney, fight kidney, pancreas, fight testis, width of stomach, length of small intestine, length of rectum and length of large intestine had not significant difference （P 〉 0.05 ）;Conclusion Different microbiological background significantly affects the hematological parameters in rabbits.

关 键 词：无菌兔 SPF兔 脏器系数 

分 类 号：Q95-331[生物学—动物学]