胰岛素对ICR小鼠胚胎体外发育的影响  

Effect of Insulin on Development of ICR Mouse Embryos in Vitro

作　　者：刘洋[1] 苏莹[1] 杜玲[1] 张媛[1] 丁芳[2] 周红林[1] 

机构地区：[1]昆明医学院第二附属医院妇科,云南昆明650101 [2]三峡大学仁和医院妇产科,湖北宜昌443001

出　　处：《昆明医学院学报》2009年第2期53-57,共5页Journal of Kunming Medical College

摘　　要：目的研究ICR小鼠胚胎体外培养时,添加胰岛素的作用、浓度及所需阶段.方法用添加不同浓度胰岛素的mKSOM培养基对小鼠胚胎进行体外培养及选择最适浓度对不同阶段鼠胚进行体外培养,观察囊胚发育率和囊胚细胞数的变化.结果所有添加胰岛素的浓度组,囊胚发育率均高于对照组,其中0.005μg/mL和0.05μg/mL浓度组囊胚细胞数显著高于对照组和其他各浓度组.2-细胞至4-细胞、4-细胞至桑椹胚前添加胰岛素囊胚率显著高于对照组和其他实验组.结论在ICR小鼠胚胎体外培养时添加胰岛素能够促进胚胎发育;胰岛素浓度增至10μg/mL对在ICR小鼠胚胎无毒性作用;在ICR小鼠胚胎体外培养的适宜胰岛素浓度为0.005μg/mL和0.05μg/mL;2-细胞至4-细胞、4-细胞至桑椹胚前添加胰岛素更加重要.Objective To study the effects and the appropriate concentration of insulin on the development of mouse embryos in vitro, and the effects of insulin on the development of different stages of embryos. Methods ICR mouse embryos were cultured in the mKSOM media supplemented with insulin at different concentrations, and the embryos during different stages were cultured with insulin at the appropriate concentration. The blastocyst rates and the cell numbers were counted. Results Additions of insulin significantly increased the rates of blastocyst and the total cell numbers. The concentration of 0.005 μg/mL, 0.05 μg/mL caused a significant increase in the total cell numbers compared with the control and other groups. Addition of insulin from 2-cell to 4-cell stage or from the 4-cell to morula stage, significantly increased the blastocyst rates compared with the control and other groups. Conclusions Insulin can promote the development of mouse embryos in vitro. The appropriate concentration of insulin added in mKSOM is 0.005 μg/mL and 0.05 μg/mL. Exposure of embryos to insulin, beginning at the 2-cell and extending to the 4-cell stage or beginning at the 4-cell and extending to the morula stage, is important for the development of ICR mouse embryos in vitro.

关 键 词：胰岛素 小鼠 胚胎 体外发育 

分 类 号：Q813.7[生物学—生物工程]