电针足三里穴对应激大鼠海马11β-羟基类固醇脱氢酶1表达的影响  

The expression of 11β-hydroxysteroid dehydrogenase type 1 in the hippocampus of rats restrained and stressed by electroacupuncture

作　　者：万顺伦[1] 尹岭[2] 

机构地区：[1]解放军第401医院神经内科,青岛266071 [2]北京解放军总医院神经信息中心

出　　处：《中华物理医学与康复杂志》2009年第3期159-162,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的观察应激大鼠海马11β-羟基类固醇脱氢酶1（11β—HSD1）的表达变化及电针足三里穴对海马11β-HSD1的调节作用，从而探讨电针调节大鼠下丘脑-垂体-肾上腺轴的可能机制。方法将实验大鼠随机分为对照组、束缚组及电针组。对照组大鼠不给予任何特殊处理；束缚组大鼠将其束缚在一个特制圆筒型鼠笼内，双后肢固定于圆筒外使其完全不能活动；电针组大鼠则在上述束缚应激状态下，取其双侧足三里穴给予电针刺激。采用Western印迹杂交法观察3组大鼠海马11β—HSD1的变化情况。结果束缚组大鼠海马内11β—HSD1表达水平明显高于对照组水平（P〈0．05）；电针组海马内11β-HSD1表达水平较束缚组进一步增加，并且持续至针刺结束后3h。结论电针足三里穴能促进束缚应激大鼠海马11β-HSD1蛋白表达水平增加，可能与负反馈调节HPA轴功能有关。Objective To observe the expression of 11 β-hydroxysteroid dehydrogenase type 1 （ 11 β-HSD1 ） in the hippocampi of rats stressed by restraint, and adjustments to 11β-HSD1 expression in response to eleetroaeupuncture. The mechanisms of adjustment of the hypothalamic-pituitary-adrenal axis （HPA） to electroaeupuneture were also studied. Methods Rats were randomly divided into a control group, a group stressed by restraint and an electroaeupuncture group. The rats in the control group received no treatment. The rats in the stressed group were put into a small columnar cage and their hind legs tied outside the cage. The electroacupuncture group, in addition to being restrained, received electroacupuncture at the Zusanli acupoints on both sides of their bodies. The rats were then sacrificed and their hippoeampi were isolated and lysed. The expression of 11 [3-HSD1 in each hippocampus were observed using the Western blotting technique. Results The levels of expression of 11β-HSD1 in the hippocampi of the restrained group were significantly higher than those in the control group. After electroacupuncture, 1 1β-HSD1 expression in the hippocampus increased further and lasted 3 h. Conclusion Electroacupuncture at the Zusanli acupoints （ST36） can increase 11β-HSD1 expression in the hippocampus of stressed rats, and this adjustment may be related to the HPA axis＇ negative feedback function.

关 键 词：海马 11Β-羟基类固醇脱氢酶1 足三里 电针 

分 类 号：R245[医药卫生—针灸推拿学]