47个亚、欧、美洲野生、地方和培育猪群的AFLP多态性及群体遗传关系研究  

The Population Genetic Relationships among 47 Chinese and Western Pig Populations Infered by AFLP Markers

作　　者：高军[1] 任军[1] Ken Siggens 艾华水[1] Gary Evens 黄路生[1] 

机构地区：[1]江西农业大学动物生物技术国家重点实验室培育基地,南昌330045 [2]Sygen molecular biology laboratory,Department of Pathology,University of Cambridge,CB2 1QP,United Kingdom

出　　处：《畜牧兽医学报》2009年第3期285-290,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目“猪基因组AFLP指纹图谱构建研究”(39860063)资助

摘　　要：采用双色荧光标记技术,检测21个中国地方猪类群、19个欧美商业猪类群、5个亚欧美野猪类群、1个中国培育猪种、1个杜洛克×中国野猪杂种群体总共47个类群基因组DNA池的AFLP多态性,22种引物组合产生312个AFLP多态标记。根据多态标记信息,计算47个类群间的遗传相似系数,并构建UPMGA聚类关系图。结果表明,中国地方猪、中国野猪与欧美商业猪群、欧美野猪间存在明显的遗传分化,提示中国地方猪起源于亚洲野猪,而欧美商业猪种由欧美野猪驯化而来。杜洛克猪、长白猪和大白猪不同类群间的遗传相似性较高,但也存在一定的遗传分化。南昌白猪与大白猪、PIC L95系与太湖猪有着很近的亲缘关系,真实反映了其育成历史。嘉兴黑猪与梅山猪、冠朝猪、藤田花猪、上犹花猪和万安花猪等地方猪群遗传关系的研究结果与其形态学、地理分布和现行分类情况相一致,认为AFLP技术是遗传多样性和群体遗传学研究的一项有效的技术手段。AFLP markers were detected by the two-color fluorescent detection in a sample of DNA pool of 21 Asian native pig populations, 19 European and American commercial pig populations, 5 wild pig populations, 1 Duroc× Chinese wild pig hybrid population and 1 Chinese sythetic pig breed. 312 polymorphic markers were detected by a total of 22 AFLP primer combinations. The genetic similarity coefficients of 47 pig populations were calculated from AFLP data, and molecular phylogenetic tree was constructed by UPMGA method. The results showed that Chinese n- ative and wild pig populations had remarkable genetic differentiation with European and American commercial pig populations and wild pig populations, indicating that Chinese native pig popu tions may originate from Asian wild pigs, while European and American commercial pig popu a- a- tions were domesticated from European and American wild pigs. Although different populations of commercial pig breeds including Duroc, Landrace and Large White had close relationship with each other, they also, to some extent, displayed genetic differentiation. Nanchang White pig and Large White, L95 dam line from PIC and Meishan pig had intimate genetic relationships with each other, which were consistent with its breeding history. Moreover, the cluster results of many other Chinese native pig breeds, including Meishan, Jiaxing Black, Guanchao, Tengtian Spotted, Shangyou Spotted and Wanan Spotted, were consistent with morphology, geographical distribution and exiting classification. It was concluded that AFLP provided a valuable tool for assaying genetic diversity and population genetic relationships in farm animals.

关 键 词：猪 AFLP 群体遗传关系 

分 类 号：S828[农业科学—畜牧学] S813.1[农业科学—畜牧兽医]