奶牛PRL基因CRS-PCR多态性与产奶性状的关联分析  

CRS-PCR Polymorphisms of Prolactin Gene and Its Relationship with Milk Production Traits in Chinese Holstein Cows

作　　者：王丽娟[1,2] 李秋玲[1] 王长法[1] 王洪梅[1] 李建斌[1] 侯明海[1] 高运东[1] 仲跻峰[1] 

机构地区：[1]山东省农业科学院奶牛研究中心,济南250100 [2]山东大学公共卫生学院,济南250012

出　　处：《畜牧兽医学报》2009年第3期303-308,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家“863”计划项目(2006AA1021D92007AA10Z169);山东省良种工程项目(2006LZ10-04);山东省农业科学院科研创新基金项目(2006YCX0282007YCX019);山东省农业科学院青年科研基金项目(2006YQN038)

摘　　要：采用CRS-RFLP技术对中国荷斯坦牛PRL基因第4外显子C8377G位点及第4内含子T8510C位点进行单核苷酸多态性分析。最小二乘分析表明:在8377位点GG基因型个体产奶量最小二乘均值显著高于CC基因型(P<0.05),GC基因型个体乳蛋白率最小二乘均值显著高于CC基因型(P<0.05);在8510位点CC基因型个体产奶量最小二乘均值显著高于TT基因型(P<0.05)。Single nucleotide polymorphisms of exon 4 C8377G and intron 4 T8510C of PRL gene were detected in Chinese Holstein cows by CRS-RFLP. At 8377 site, the least square mean of milk yield of this population was higher for individuals with genotype GG than that for individuals with genotype CC （P〈0.05） ; The least square mean of protein percentage of this population was higher for individuals with genotype GC than that for individuals with genotype CC （P〈0. 05）. At 8510 site, the least square mean of milk yield of this population was higher for individuals with genotype CC than that for individuals with genotype TT （P〈0.05）.

关 键 词：中国荷斯坦牛 催乳素基因 产奶性状 SNPS CRS-PCR 

分 类 号：S828[农业科学—畜牧学] S852.659.5[农业科学—畜牧兽医]