STAT1基因单核苷酸多态性与中国荷斯坦奶牛产奶性状的相关性研究  

Association between Polymorphism of STAT1 Gene and Milk Production Traits in Chinese Holstein Cattle

作　　者：褚敏[1] 昝林森[1] 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100

出　　处：《畜牧兽医学报》2009年第3期309-314,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："十一五"国家科技支撑计划"西北地区生态型奶业生产技术和奶牛性控技术研究与开发"(2006BAD04A11);陕西省重大科技专项"肉牛;奶牛优质高效养殖关键技术研究与示范"(2006K207-G1);西北农林科技大学拔尖人才支持计划项目

摘　　要：采用PCR-SSCP方法检测199头中国荷斯坦奶牛STAT1基因内含子11、19、25及3-′UTR 4个位点的单核苷酸多态性,并将其与3个胎次的产奶性状进行相关性分析。结果在内含子11和3-′UTR 2个位点发现多态性,在内含子11中发现2个单核苷酸连锁突变,分别为28739碱基处发生的G→A转换,28873碱基处发生的G→C颠换;在3-′UTR 141930碱基处发现C→T突变。利用最小二乘分析研究3个位点不同基因型对中国荷斯坦奶牛3个胎次305天产奶量,乳脂率及乳蛋白率的影响。结果表明,在内含子11中,BB基因型个体第一、二胎次乳蛋白率均显著高于AA和AB基因型个体(P<0.05);3种基因型对各胎次产奶量及乳脂率的影响均未达到显著水平(P>0.05),但呈现出BB>AB>AA的趋势。在3-′UTR中,CC、CT基因型个体的第一、二胎次产奶量均显著高于TT基因型个体(P<0.05);CC基因型个体的第一、三胎次乳蛋白率均显著高于TT基因型个体(P<0.05),3种基因型对3个胎次乳脂率的影响差异均不显著(P>0.05),但呈现出CC>CT>TT的趋势。The polymorphisms in intron 11,19,25 and 3＇-UTR of STAT1 gene were detected in 199 Chinese Holstein eattles with PCR-SSCP and association between the polymorphisms and milk production traits of three birth orders were analyzed. There were three polymorphism sites in Chinese Holstein eattles, which were in intronll and 3＇-UTR. We found that it was G/A （28739） and G/C （28873） in intron 11, and C/T （141930） in 3＇-UTR. The association between different genotypes of three sites and 305 days milk yield, fat percent （F%）, and protein percent （P %） of three birth orders was analyzed by least square analysis. The results showed that in intron ll,there were no significant differences among three genotypes on 305 days milk yield, and fat percent（F%）of all birth orders（P〉0.05）, but the tendency of BB〉AB〉AA was showed. Cows with genotype BB had significantly higher protein percent （P%） in the first and second birth orders （P〈0.05） than those with AB and AA. And in 3＇-UTR, there were no significant differences among three genotypes on fat percent （F%） （P〈0. 05）, while the tendency of CC〈 CT〈TT was showed either. And cows with genotype CC or CT had significantly higher 305 days milk yield in the first and second birth orders （P^0.05） ; and cows with genotype CC had significantly higher protein percent （P%） than those with genotype TT in the first and third birth orders（P〈0.05）.

关 键 词：STAT1基因 PCR-SSCP 荷斯坦奶牛 产奶性状 

分 类 号：S823.912[农业科学—畜牧学] Q987[农业科学—畜牧兽医]