猪GPR54基因在下丘脑、垂体、卵巢发育性表达变化的研究  

Study on the Change of GPR54 Gene Development Expression in Hypothalamus,Pituitary and Ovaries in Pigs

作　　者：周春宝[1,2] 汪劲能[1] 陆艳凤[1] 丁家桐[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏畜牧兽医职业技术学院,泰州225300

出　　处：《畜牧兽医学报》2009年第3期333-337,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省自然科学基金(2005050)

摘　　要：分别随机选取处于初生、60日龄、120日龄、初情期、180日龄的苏姜猪母猪各4头,进行屠宰,采集下丘脑、垂体、卵巢,采用反转录多聚酶链式反应(RT-PCR),以-βactin作内标,定量分析下丘脑、垂体、卵巢中GPR54mRNA发育性变化。结果显示:苏姜猪GPR54 mRNA在下丘脑、垂体、卵巢组织内从初生到初情期表达量逐渐上升,初情期后呈下降趋势,初情期GPR54 mRNA表达丰度与初生、180日龄差异显著(P<0.05);在不同组织器官中,GPR54 mRNA表达丰度在卵巢中最高,下丘脑中表达丰度最低,下丘脑的表达丰度与垂体、卵巢中的表达丰度均差异显著(P<0.05)。Four female Sujiang pigs were randomly selected at different stages of birth, 60 days, 120 days, puberty and 180 days, respectively and slaughtered, tissue samples of hypothalamus, pituitary and ovaries were collected. Development expression of GPR54 mRNA which was related to puberty before and after in hypothalamus, pituitary and ovaries were determined by quantita- tive RT-PCR with 13-aetin as an internal control. The results showed that the expression abundance of GPR54 mRNA increased gradually from birth to puberty, and after puberty the expression abundance had degressive tendency. At different stages of development, the expression abundance at the stage of puberty was significantly different with that at the stages of birth and 180 days（P〈0.05）. In different tissues, the expression abundance was highest in ovary and lowest in hypothalamus, the expression abundance in hypothalamus was significantly different with those in ovary and pituitary（P〈0.05）.

关 键 词：猪 GPR54 初情期 克隆 荧光定量PCR 

分 类 号：S814[农业科学—畜牧学] S828[农业科学—畜牧兽医]